Description: Mannose receptor, Human, mAb 15-2

The monoclonal antibody 15-2 recognizes the mannose receptor (MR), also known as CD206, a member of the vertebrate C-type lectin family. The mannose receptor, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 175 kDa single-pass type I transmembrane receptor consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail.The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR binds high-mannose structures on- a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator.MRs are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines as IFN-gamma. Labeling of MR expressing monocytes/macrophages increases with prolonged incubation time probably due to internalization of the MR-antibody-complex. The monoclonal antibody 15-2 prevents binding of glycoproteins including t-PA to MR.Detection of the MR with anti-MR monoclonal antibody 15-2 can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.

Specifications:

Catalog number Product type Quantity Formulation Application Application Notes Use Immunogen Isotype Species Alias Positive Control Negative Control References Storage and stability Precautions Disease

HM2056

Monoclonal antibodies

100 µg

1 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin. The endotoxin concentration is < 24 EU/mg, determined with HIT302 LAL Assay.

Flow cytometry, Frozen sections, Functional studies, Western blot

F: Tissue sections were fixed in acetone/chloroform. Sections were preincubated with normal rabbit serum (Ref.3).FC: Antibody 15-2 stains the extracellular domain of MR.W: A non-reduced sample treatment and 6% SDS-Page was used. The band size is 175 kDa (Ref.1).FS: Antibody 15-2 functions as an inhibitor of binding. The antibody was functionally tested by preincubation of immobilized receptor with antibody. This prevented binding of t-PA to immobilized receptor. (Ref.1).

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Purified human mannose receptor from human placental tissue

Mouse IgG1

Human

Machrophage Mannose Receptor 1, MRC1, CLEC13D, MMR, C-type lectin domain family 13 member DGene name: MRC1

Macrophages (in coronary atherosclerotic plaque), placenta tissue, Hofbauer cells (fetal macrophages), liver tissue

Lymphocytes, Monocytes

1. Barret-Berghoeff, M et al; Monoclonal antibodies against the human mannose receptor that inhibit the binding of tissue-type plasminogen activator. Thromb Haemostas 1997,77: 7182. Noorman, F et al; Monoclonal antibodies against the human mannose receptor as a specific marker in flow cytometry and immunohistochemistry for macrophages. J Leukocyte Biol 1997, 61: 633. Noorman, F et al; Cluster mannosides can inhibit mannose receptor-mediated tissue-type plasminogen activator degradation by both rat and human cells. Hepatology 1997, 26: 13034. Tailleux, L et al; DC-SIGN is the major mycobacterium tuberculosis receptor on human dendritic cells. J Exp Med 2003, 197: 1215. Pressice, P et al; Keyhole limpet hemocyanin induces the activation and maturation of human dendritic cells through the involvement of mannose receptor. Mol Immunol 2008, 45: 11366. Tahara, N et al; 2-deoxy-2-[(18)F]fluoro-d-mannose positron emission tomography imaging in atherosclerosis. Nat Med 2014, 20: 215

Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.

Cardiology and metabolism, Infectious diseases

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