Description: Apoptotic Neutrophils, Human, mAb BOB93

The monoclonal antibody BOB93 binds to the surface of apoptotic neutrophils. Neutrophils have been implicated in the pathogenenesis of a variety of inflammatory diseases including the adult respiratory distress syndrome, idiopathic pulmonary fibrosis, ulcerative colitis and rheumatoid arthritis. Although the neutrophil is a vital component of the bodys defense against infectious agents, uncontrolled release of its toxic substances may inflict \"friendly fire\" damage on surrounding tissue and propagate the inflammatory response, leading to scarring and tissue destruction. Apoptosis leads to recognition and safe disposal of dying cells by phagocytosis. Binding of the monoclonal antibody BOB93 to apoptotic neutrophils is dependent on the presence of sialoglycoprotein fetuin, a constituent of bovine serum. Fetuin is the antigen for BOB93, and BOB93 and fetuin form a complex in solution that is necessary and sufficient for binding to apoptotic neutrophils. The antigen recognised by BOB93 may act as a \"molecular bridge\" between the surface of the apoptotic neutrophil and the phagocyte to modulate apoptotic neutrophil clearance at inflammatory sites. Human macrophage phagocytosis of apoptotic neutrophils was augmented in vitro by addition of the antigen recognised by BOB93, which does not bind to other apoptotic leukocytes.

Specifications:

Catalog number Product type Quantity Formulation Application Use Isotype Species References Storage and stability Precautions Disease

HM2154

Monoclonal antibodies

100 µg

1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.

Flow cytometry, Western blot

For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Mouse IgG1

Human

Hart, S et al; Specific binding of an antigen-antibody complex to apoptotic human neutrophils. Am J Pathol 2003, 162: 1011

Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Cardiology and metabolism, Neurological disorders

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