Description: CD180, Human, mAb MHR73

The monoclonal antibody MHR73 reacts with human RP105 (CD180). RP105 is a type 1 transmembrane protein of 105 kDa with extracellular leucine-rich repeats (LLRs) and a short cytoplasmic tail. RP105, assigned as CD180, is similar to Drosophila Toll in the extracellular LLRs. RP105 expression is largely restricted to immune cells including CD19+ B cells and macrophages. Histological studies showed that RP105 is expressed mainly on mature B cells in mantle zones. Antibody-mediated (MHR73) cross-linking of RP105 induces resistance against irradiation-induced apoptosis, B-cell proliferation, and up-regulation of a costimulatory molecule CD80, revealing RP105 as a potent regulator of B-cell activation. In this context it is important to note that RP105 is associated with MD1, and that it mediates B-cell surface expression and LPS recognition and signalling in cooperation with TLR4. MD-1 seems to be requisite for efficient expression of RP105.

Specifications:

Catalog number Product type Quantity Formulation Application Use Isotype Species References Storage and stability Precautions Disease

HM2083

Monoclonal antibodies

100 µg

1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS containing 0.1% bovine serum albumin.

Flow cytometry, Frozen sections, Functional studies, Immuno precipitation, Paraffin sections

For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For neutralization of biological activity dilutions have to be made according to the amounts RP150 to be inactivated.

Mouse IgG1

Human

1. Miura Y, et al; RP105 is associated with MD-1 and transmits an activation signal in human B cells. Blood 1998, 92: 2815 2. Tsuneyoshi, N et al; The functional and structural properties of MD-2 required for lipopolysaccharide binding are absent in MD-1. J Immunol 2005, 174: 340

Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year.

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.

Autoimmunity, Tumor immunology

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