Description: C5L2, Rat, pAb
The polyclonal antibody reacts with rat C5L2. The orphan receptor C5L2 is a seven transmembrane receptor with 40% amino acid sequence identity to the C5a receptor, CD88. Expression has been found on granulocytes and immature dendritic cells. In contrast to CD88, C5L2 is uncoupled from G proteins due to an an amino acid replacement of arginine by leucine in the so called DRY region at the end of the third intracellular transmembrane domain. Following C5a binding, C5L2 seems neither to induce classical signalling nor to cause biological responses. When C5L2 was transfected into several cell types, C5a failed to induce chemotaxis, degranulation, or interacellular calcium mobilization. The high affinfity of C5L2 for C5a and its metabolite, C5adesArg and a low affinity for C3a and C3adesArg suggest that this receptor is a scavenger of complement fragments involved in the defence against the harmful side effects of complement activation. C5L2 expression on human neutrophils is known to be decreased in sepsis; loss of expression may be a prognostic indicator in this condition as survivors of sepsis tend to have higher C5L2 levels.Specifications:
| HP8018 |
| Polyclonal antibodies |
| 100 µg |
| 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin. |
| Flow cytometry, Functional studies |
| For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. |
| Rabbit Ig |
| Rat |
| 1. Cain, S et al; The orphan receptor C5L2 has high affinity binding sites for complement fragments C5a and C5a des-Arg74. J Biol Chem 2002, 277: 7165 2. Kalant, D et al; The chemoattractant receptor-like protein C5L2 binds the C3a des-Arg77/acylation-stimulating protein. J Biol Chem 2003, 278: 11123 3. Huber-Lang, M et al; Changes in the novel orphan, C5a receptor (C5L2), during experimental sepsis and sepsis in humans. J Immunol 2005, 174: 1104 |
| Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year. |
| For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product. |
| Infectious diseases, Nephrology |
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