Description: PAI-1, Human, mAb MA-56A7C10
Plasminogen activator inhibitor type-1 (PAI-1), a member of the serine protease inhibitor (serpin) superfamily is an important protein in the regulation of fibrinolysis. PAI-1 is unique among the serpins because of its functional and conformational flexibility. PAI-1 is the most important physiological inhibitor of both tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (u-PA). Increased PAI-1 levels are associated with thrombotic events and is an established risk factor for cardiovascular diseases. The active conformation PAI-1 inhibits its target proteinases by the formation of a stable, inactive complex. Although PAI-1 is synthesized as an active molecule, it converts spontaneously to an inactive, latent form that can be partially reactivated by denaturing agents. In addition, a third conformation reacting as a non-inhibitory substrate towards various target proteinases has been identified. The residues Glu242, Lys243 and Glu244, located in the loop connecting s3B with hG (designated loop 2) and the residues Glu350, Asp355 and Arg356, located at the distal hinge of the reactive site loop, form the major site of interaction for the monoclonal antibody MA-56A7C10. This is clearly a conformational epitope comprising residues clustered together in the three dimensional structure. MA-56A7C10 exhibits differential reactivity toward the different conformations of human PAI-1 The monoclonal antibody MA-56A7C10 generates a non-reactive form of PAI-1, whether this is the latent form or that the reactive site is blocked by the antibody is unknown. The antibody cross reacts with porcine PAI-1.Specifications:
| HM2181 |
| Monoclonal antibodies |
| 100 µg |
| 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin. |
| Functional studies, Immuno assays |
| For inhibition of biological activity dilutions have to be made according to the amounts PAI-1 to be inactivated. |
| Mouse IgG1 |
| Human |
| Pig - Yes |
| Bijnens, A et al; The distal hinge of the reactive site loop and its proximity. J Biol Chem 2001, 276: 44912 |
| Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year. |
| For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product. |
| Cardiology and metabolism, Tumor immunology |
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