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商品描述
Description:MASP-2,Human,ELISAkit
MASP-2isaMBLassociatedserineprotease.Mannan-bindinglectin(MBL)andficolins,incomplexwithMASPs,arecapableofactivatingthecomplementsystem,thusmediatingthedestructionofinfectiousagents.AfteroligomerisationofMBL(orL-Ficolin)MASP-2willbeboundandactivated.ActivatedMASP-2cleavesC4andC2andiscrucialfortheactivationofdownstreamcomplementcomponents.MASP-2deficiencywillresultinanincreasedsusceptibilityforinfections(e.g.leukemiapatientsinchemotherapyareathighriskofseriousinfections).EvenmoresothenMBLdeficiency,becauseMASP-2isalsorequiredforcomplementactivationbyH-andL-ficolin.TheELISAdetectsMASP-2inserumandplasma.TheMASP-2levelsinplasmafromhealthyindividualsrangefrom170to1196ng/ml.
Specifications:
| Catalognumber | HK326-01 |
|---|---|
| Producttype | Assays |
| Quantity | 1x96det. |
| Standardrange | 1.6-100ng/ml |
| Detectionlevel | 1.6ng/ml |
| Workingvolume | 100µl/well |
| Species | Human |
| Application | ThehumanMASP-2ELISAkitistobeusedfortheinvitroquantitativedeterminationofhumanMASP-2inserum,plasmaandcellculturesupernatantsamples. |
| Disease | Infectiousdiseases |
| Principle | ThehumanMASP-2ELISAisaready-to-usesolid-phaseenzyme-linkedimmunosorbentassaybasedonthesandwichprinciplewhichaworkingtimeof3½hours.Theefficientformatof2plateswithtwelvedisposable8-wellstripsallowfreechoiceofbatchsizefortheassay.Samplesandstandardsarecapturedbyasolidboundspecificantibody.BiotinylatedtracerantibodywillbindtocapturedhumanMASP-2.Streptavidin-peroxidaseconjugatewillbindtothebiotinylatedtracerantibody.Streptavidin-peroxidaseconjugatewillreactwiththesubstrate,tetramethylbenzidine(TMB).Theenzymereactionisstoppedbytheadditionofoxalicacid.Theabsorbanceat450nmismeasuredwithaspectrophotometer.Astandardcurveisobtainedbyplottingtheabsorbance(linear)versusthecorrespondingconcentrationsofthehumanMASP-2standards(log).ThehumanMASP-2concentrationofsamples,whicharerunconcurrentlywiththestandards,canbedeterminedfromthestandardcurve. |
| Linearity | Thelinearityoftheassaywasdeterminedbyseriallydilutingasamplecontaining328ng/mlhumanMASP-2.Thedilutedsamplesweremeasuredintheassay.Thelineobtainedaslopeof1.01andacorrelationcoefficientof0.999. |
| StorageandstABIlity | Productshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Thekitistobeusedforresearchpurposesonly,notforclinicalordiagnosticuse.TheMASP-2ELISAisPatentprotectedbypatent09/874,238andPCT/DK2004/000338.LicenserightsonthePatentsareacquiredfromNatimmuneS/A,Denmark.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
| References | 1.Møller-Kritensen,Metal;Levelsofmannan-bindinglectin-associatedserineprotease-2inhealthyindividuals.JImmunolMeth2003,282:159 2.Schlapbach,Letal;Deficiencyofmannose-bindinglectin-associatedserineprotease-2associatedwithincreaseriskoffeverandneutropeniainpediatriccancerpatients.PedInfectDis2007,26:989 3.Garcia-Laorden,Metal;Mannose-bindinglectinandmannose-bindinglectin–associatedserineprotease2insusceptibility,severity,andoutcomeofpneumoniainadults.JAllergyClinImmunol2008,122:368 4.Zehnder,Aetal;Prognosisinpediatrichematologicmalignanciesisassociatedwithserumconcentrationofmannose-bindinglectin-associatedserineprotease-2(MASP-2).PediatrBloodCancer2009,53:53 5.Schlapbach,Letal;HigherCordBloodLevelsofMannose-BindingLectin-AssociatedSerineProtease-2inInfantswithNecrotisingEnterocolitis.PediatricRes2008,64:562 |
