Description:

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Murine JE/MCP-1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Murine JE/MCP-1 proteins within the range of 63-4000 pg/ml. The Murine JE/MCP-1 ELISA is capable of recognizing both recombinant and naturally produced Murine JE/MCP-1 proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.• Rat MCP-1The antigens listed below were tested at 50 ng/ml and exhibited less than 2% cross reactivity. • Murine MCP-3The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity. • Human MCP-1, MCP-3• Murine MCP-5The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human CTACK, MCP-2, MIP-4, MIP-1beta• Murine C10, CTACK, Eotaxin, KC, MCP-2, MDC, MIP-1alpha, MIP-1beta, MIP-1gamma, MIP-3beta, RANTES

Additional Information:

Name Related Product Names Gene Symbol Gene Fullname Gene id Alias Symbols Protein Name Description of Target Swissprot ID Protein Accession Num Nucleotide Accession Num Species Reactivity Assay Info Sensitivity Kit Range Standard Range Sample Type Product Format Specimen/Volumes Swiss Prot Number Specificity Applications GI Number Format Stability Reconstitution and Storage Reactivity Kit Size Kit Principle Kit Component Storage Lead Time Intended Use

Mouse JE/MCP-1 ELISA Kit

C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Platelet-derived growth factor-inducible protein JE, Small-inducible cytokine A2, Scya2

CCL2

chemokine (C-C motif) ligand 2

20296

AI323594, C-C motif chemokine 2, HC11, Je, JE, MCAF, Mcp1, MCP1, MCP-1, Monocyte chemoattractant protein 1, monocyte chemoattractant protein-1, monocyte chemotactic protein, Monocyte chemotactic protein 1, Platelet-derived growth factor-inducible protein

C-C motif chemokine 2

This gene is one of several cytokine genes clustered on chromosome 11. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of

P10148

NP_035463.1

NM_011333.3

Mouse

Quantitative Colorimentric Sandwich ELISA

63 pg/mL

63-4,000 pg/mL

63-4000 pg/ml

Cell lysates, sera and plasma

1 x 96-Well Plate or 12 x 8-Well Strips

Refer to Manual

P10148

The Murine JE/MCP-1 ELISA is capable of recognizing both recombinant and naturally produced Murine JE/MCP-1 proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.• Rat: MCP-1The antigens listed below were tested at 50 ng/ml and exhibited less than 2% cross reactivity.• Murine: MCP-3The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity.• Human: MCP-1, MCP-3• Murine: MCP-5The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.• Human: CTACK, MCP-2, MIP-4, MIP-1β• Murine: C10, CTACK, Eotaxin, KC, MCP-2, MDC, MIP-1α, MIP-1β, MIP-1γ, MIP-3β, RANTES

ELISA

20296

ELISA Kit. Refer to manual for specific component formats

6 months

4C/6 Months

Mouse

12 x 8-Well Microstrips

The Aviva Murine JE/MCP-1ELISA Kit contains the components necessary for quantitative determination of natural or recombinant mJE/MCP-1concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a \"Sandwich\" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a \"sandwich\" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator.The capture antibodies coated to the bottom of each well are specific for a particular epitope on theMurineJE/MCP-1cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and \"sandwiching\" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetricreaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

ComponentsAmount Capture Antibody Coated Microplate 96 Wells (12 x 8) Ready-to-Use Streptavidin-HRP 12 mL Ready-to-Use Substrate 12 mL Stop Solution 12 mL Wash Buffer (15x) 50 mL Protein Standard Diluent 12 mL Sample Diluent 12 mL Detection Antibody Diluent 12 mL Biotin-Labeled Detection Antibody Lyophilized Protein Standard Lyophilized (100 ng)

4°C/6 Months

Domestic: within 1 week deliveryInternational: 1 week

Research Use Only

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