Product Name| Human Placental Lactogen. |
Category| Fertility. |
Sample Type| Serum. |
Sensitivity| 0,3 mg/mL. |
Description| Enzyme Immunoassay Kit provides materials for the Quantitative Determination of human Placental Lactogen (hPL) in serum. |
# of Samples| 1X96. |
Introduction| The GenWay hPL Enzyme Immunoassay Kit provides materials for the quantitative determination of human Placental Lactogen (hPL) in serum.This assay is intended for research use only.The physiological role of HPL has not yet been established, but the great similarity to human growth hormone has stimulated the hypothesis about a function as a regulator of feto-placental growth and other physiological alterations during pregnancy. It has been suggested that the maternal serumlevel may reflect on \"index of placental function\".Depressed levels of HPL are seen in association with intrauterine death, fetal distress in labor and birth asphyxia. This association is particularly strong if depressed levels are seen repeatedly, implying a chronic state of placental and therefore fetal compromise. Depressed levels are usually not present if the pregnancy has proceeded uneventfully to term.Elevated levels of HPL are usually indicative of optimal pregnancy outcome in singleton pregnancies. However, high levels may indicate substantial fetal pathology in specific diseases, namely diabetes mellitus and fetal macrosomia, rhesus isoimmunization and hydrops fetalis. |
Principle of the test| The Biosource hPL ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle.The microtiter wells are coated with a monoclonal antibody directed towards a unique antigenic site on an hPL molecule. An aliquot of experiemental sample containing endogenous hPL is incubated in the coated well with enzyme conjugate, which is a monoclonal anti-hPL antibody conjugated with horse radish peroxidase. After incubation the unbound conjugate is washed off.The amount of bound peroxidase is proportional to the concentration of hPL in the sample.Having added the substrate solution, the intensity of colour developed is proportional to the concentration of hPL in the experimental sample. |
Storage and Stability| When stored at 2°C-8°C unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.Opened reagents must be stored at 2°C-8°C. Microtiter wells must be stored at 2°C-8°C. Once the foil bag has been opened, care should be taken to close it tightly again.Opened kits retain activity for six weeks if stored as described above. |
Function| Similar to that of somatotropin. |
Subcellular Location| Secreted. |
Miscellaneous| The sequence of CSH1 is shown. |
Similarity| Belongs to the somatotropin/prolactin family. |
