Description:
Product Name: ProlactinCategory: FertilitySample Type: SerumSensitivity: 11 IU/mLDescription: Immunoenzymetric assay for the in vitro quantitative measurement of human Prolactin (PRL) in serum and plasma.# of Samples: 15X9Intended Use: Immunoenzymetric assay for the in vitro quantitative measurement of human Prolactin (PRL) in serum and plasma.Background: A. Biological activitiesProlactin (PRL) is a polypeptide hormone (molecular weight 20,000 Da) secreted by the pituitary gland, which plays a key role in the development of the mammary gland, the production and secretion of milk and the control of male and female gonadal functions. Prolactin secretion is under hypothalamic control exerted directly by dopamine, several prolactin releasing factors (PRF) and perhaps VIP (vasoactive intestinal polypeptide) or a closely related peptide. TRH also acts directly at the pituitary level to stimulate prolactin release but its physiological role in the control of prolactin secretion has not been established yet. Several neuroendocrine factors, involving serotoninergic or noradrenergic pathways are also involved in the control of prolactin secretion. The plasma concentration of prolactin increases in various physiological situations such as stress, pregnancy and lactation. Physiological levels fluctuate according to a nycthemeral rhythm, a significant rise being observed at night. Drugs with anti-dopamine activity (psychotropic agents) and ovulatory suppressants, increase prolactin secretion.Principles of the method: The GenWay PRL-EASIA is a solid phase Enzyme Amplified Sensitivity Immunoassay performed on microtiterplate. The assay uses monoclonal antibodies (MAbs) directed against distinct epitopes of Prolactin. Calibrators and samples react with the capture monoclonal antibody (MAb 1) coated on microtiter well and with a monoclonal antibody (MAb 2) labelled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich: coated MAb 1 - human Prolactin - MAb 2 - HRP, the microtiterplate is washed to remove unbound enzyme labelled antibody. Bound enzymelabelled antibody is measured through a chromogenic reaction. Chromogenic solution (TMB) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance, which is proportional to the Prolactin concentration.A calibration curve is plotted and PRL concentration in samples is determined by interpolation from the calibration curve. The use of the EASIA reader (linearity up to 3 OD units) and a sophisticated data reduction method (polychromatic data reduction) result in a high sensitivity in the low range and in an extended calibration range.Storage and Stability: Before opening or reconstitution, all kits components are stable until the expiry date, indicated on the vial label, if kept at 2 to 8C. Unused strips must be stored, at 2-8C, in a sealed bag containing a desiccant until expiration date. After reconstitution, calibrators and controls are stable for 1 week at 2 to 8C. For longer storage periods, aliquots should be made and kept at -20C for maximum 3 months. Avoid successive freeze thaw cycles. The concentrated Wash Solution is stable at room temperature until expiration date. Freshly prepared Working Wash solution should be used on the same day. After its first use, the conjugate is stable until expiry date, if kept in the original well-closed vial at 2 to 8C. The freshly prepared revelation solution is stable, before use, for maximum 15 minutes at room temperature and must be discarded afterwards. Alterations in physical appearance of kit reagents may indicate instability or deterioration.Sensitivity and Specificity: The Cross-reactivity of LH, FSH, hCG, hPL, TSH and hGH was determined by addition of each analyte to serum samples containing respectively 116 ?IU/ml and 2539 ?IU/ml of PRL. The apparent PRL concentration was measured. As shown below, the cross-reactions with LH, FSH, hCG, hPL, TSH and hGH are insignificantProduct Name: Prolactin Function: Prolactin acts primarily on the mammary gland by promoting lactation.Subcellular Location: Secreted.Similarity: Belongs to the somatotropin/prolactin family.
Additional Information:
| Prolactin ELISA Kit |
| Prolactin ELISA Kit; PRL ProlactinPR |
| 25876 |
| P01236 |
| Research Use Only |
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