Description:

Description: Human Kit (V654A) ( GenBank Accession No. NM_000222), (a.a. 544- 976) with N-terminal His tag, MW=53.4 kDa, expressed in an Baculovirus infected Sf9 cell expression system. Source: Baculovirus infected Sf9 cells Stability: >6 months at -80°C Purity: >20% by SDS-PAGE. Specific Activity: XX U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Naorthovanadate, 0.1 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 4 ug /ml recombinant cKit. Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Host: baculovirusTag: N-terminal His-tagGI Num: NM_000222MW: 53.4 kDaFormat: aqueous buffer solutionFormula: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazoleScientific Category: Kinase/Receptor TyrosineSpecies: human

Additional Information:

Name Related Product Names Molecular Weight Purity Source GI Number Format Reactivity Storage Intended Use

cKit (V654A), Active Human Recombinant Protein

cKit (V654A), Active Human Recombinant Protein

53.4 kDa

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

baculovirus

NM_000222

Aqueous Buffer Solution

Human

-80°C

Research Use Only

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