GenWay/Sirtuin 2, Active Human Recombinant Protein/GWB-A5C283/100µg-蚂蚁淘在线

Description: Human Sirtuin 2, GenBank Accession No. NM_030593, amino acids 13-319 with C-terminal His tag, MW= 35.5 kDa, expressed in E. coli expression system. Specific Activity : 1.4 U/µg. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate, Catalog number 50032, 500 µM NAD+, and 100 ng/μl Sirtuin 2. Incubation condition: 30 min at 37°C. Application : Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Stability :>6 months at -80°C. References : 1. Grubisha, O., 2006, J.Biol.Chem. 281, 20, 14057-14065 2. Vaquero, A., 2006, Genes Dev. 20, 10, 1256- 1261 Assay Protocol Material : Assay buffer, BPS catalog number 50031; Assay developer, BPS catalog number 50030; HDAC Substrate, BPS number 50032, Step 1 : Adding all reaction mixture to a low binding black plate 20 µl of Sirtuin 2, 0.1 ug/ul, 15 µl of HDAC assay buffer, BPS catalog number 50031, 5 µl of 1 mg/ml BSA 5 µl of 2 mM NAD+ 5 µl of 200 uM substrate, BPS catalog number: 50032, 20 µl of Sirtuin 2, 0.1 ug/ul, Always add the enzyme at the last. Incubate at 37 C for 30 min. Step 2 : Stop the reaction Add 50 µl of HDAC assay developer, 2x, , BPS catalog number 50030, and incubate the plate at room temperature for 15 min Step 3 : Read sample in a microtiter-plate reading fluorimeter capable of excitation at a wavelength in the range 350-380 nm and detection of emitted light in the range 440-460 nm. Host: E. coliTag: C-terminal His tag GI Num: NM_030593MW: 35.5 kDaFormat: aqueous buffer solutionFormula: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 200 mM imidazoleScientific Category: Deacetylase/EnzymesSpecies: human