Description:

Description: Myeloperoxidase (MPO) is a peroxidase enzyme (EC 1.11.1.7) most abundantly present in neutrophil granulocytes. It is a green hemoprotein found in neutrophils and monocytes that catalyzes the reaction of hydrogen peroxide and halide ions to form cytotoxic acids and other intermediates that play a role in the oxygen-dependent killing of tumor cells and microorganisms. Its heme pigment causes the green color in secretions rich in neutrophils, such as pus and some forms of mucus. Furthermore, it can oxidize tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent. In GenWays MPO Assay Kit, MPO catalyzes the production of NaClO from H2O2 and NaCl. Subsequently, NaClO will react stoichiometrically with Aminophenyl fluorescein (APF) to generate fluorescein, which has a strong fluorescence and can be detected at Ex/Em = 485/525 nm. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput assay of MPO activity. This kit can be used to detect MPO activity as low as 0.5 µU per well.Summary: - Detection method- Fluorescence (Ex/Em = 485/525 nm)- Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc.- Species reactivity- Mammalian Kit components: - Assay Buffer- MPO Substrate Stock- MPO Probe- Fluorescein Standard (1 mM)- MPO Positive ControlFeatures and Benefits: - Simple procedure- Fast and convenient- Sensitive assays for measuring MPO as low as 0.5 µU per wellShipping Conditions: Gel pack

Additional Information:

Name Related Product Names Format Storage Intended Use

Myeloperoxidase (MPO) Fluorometric Activity Assay Kit

Myeloperoxidase , Myeloperoxidase kit, Myeloperoxidase assay, MPO, MPO kit, MPO assay, MPO Fluorometric assay, Fluorometric MPO assay

Assay Kit

-20C

Research Use Only

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