Description:

pOET5isadualpromoterbaculovirustransfervectordesignedforhighlevelexpressionoftwoforeigngenessimultaneouslyunderthepowerfulAcMNPVpolyhedron(polh)promoterandtheverylatep10promoter.Thepromotersareinoppositeorientationstominimizerecombination.Thevectorissmallerthanotheravailabletransfervectors(4590bp)whichgreatlyfacilitatethecloningsteps.IthasabacterialoriginofreplicationandanampicillinresistancegeneforselectioninE.coli.Thepolhsequenceshavebeenreplacedbytwomultiplecloningsitescontaininguniquerestrictionsitesforinsertionoftheforeigngenesinthecorrectorientation.TheAcMNPVsequencesflankingthegeneinthetransfervectorsMCSallowrecombinationwiththeviralDNAtoinserttheexpressioncassetteintothepolhlocus.pOET5iscompatIBLewithanybaculovirussystemthatutilizeshomologousrecombinationininsectcells.



AdditionalInformation:

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Name	pOET-5TransferVector(10ug)
Concentration	LotSpecific
StABIlity	1year
Storage	-20C
IntendedUse	ResearchUseOnly