Description:

pOET2C_6xHisisabaculovirustransfervectordesignedforhighlevelexpressionofforeigngenesunderthepowerfulAcMNPVpolyhedron(polh)promoter. ThevectorencodesanoptionalC-terminal6×His-Tag^® fusionsequencethatmaybeutilized. Thisgreatlyeasesthepurificationoftherecombinantproteinsincethe6×His-containingfusionproteinsbindwithhighaffinitytoNi-NTAAgarose. pOET2C_6xHishasaColE1originofreplicationandanampicillinresistancegeneforselectionin E.coli.Thepolh sequenceshavebeenreplacedbyamultiplecloningsite(MCS)containinguniquerestrictionsitesforinsertionoftheforeigngeneinthecorrectorientation,asshownonthecircularmap. ThecodingstrandoftheMCSastranscribedfromthe polh promoterisshownbelowthecircularmap. TheAcMNPVsequencesflankingthegeneinthetransfervectorsMCSallowrecombinationwiththeviralDNAtoinserttheexpressioncassetteintothe polh locus.pOET2C_6xHisiscompatIBLewithanybaculovirussystemthatutilizeshomologousrecombinationininsectcells.

AdditionalInformation:

GenWay为每个蛋白质表达项目提供定制的解决方案。我们专业的科研人员对困难的蛋白质具有丰富的经验，并已生产出2500多种重组蛋白质。无论您的需求是小型研究还是大型cGMP，我们都可以运用我们的经验来满足您的需求。服务包括大肠杆菌表达，哺乳动物表达，酵母表达和抗原制备。如果您需要报价或安排电话讨论您的需求，请使用我们方便的在线蛋白质表达报价表。

Name	pOET-2C6xHistransferplasmid
Concentration	LotSpecific
StABIlity	1year
Storage	-80C
IntendedUse	ResearchUseOnly