Description:

pOET1C_6xHisisabaculovirustransfervectordesignedforhighlevelexpressionofforeigngenesunderthepowerfulAcMNPVpolyhedron(polh)promoter.ThevectorencodesanoptionalC-terminal6×His-Tagfusionsequencethatmaybeutilized.Thisgreatlyeasesthepurificationoftherecombinantproteinsincethe6×His-containingfusionproteinsbindwithhighaffinitytoNi-NTAAgarose.pOET1C_6xHishasaColE1originofreplicationandanampicillinresistancegeneforselectioninE.coli.Thepolhsequenceshavebeenreplacedbyamultiplecloningsite(MCS)containinguniquerestrictionsitesforinsertionoftheforeigngeneinthecorrectorientation,asshownonthecircularmap.ThecodingstrandoftheMCSastranscribedfromthepolhpromoterisshownbelowthecircularmap.TheAcMNPVsequencesflankingthegeneinthetransfervectorsMCSallowrecombinationwiththeviralDNAtoinserttheexpressioncassetteintothepolhlocus.pOET1C_6xHisiscompatIBLewithanybaculovirussystemthatutilizeshomologousrecombinationininsectcells. 

AdditionalInformation:

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Name	pOET1C6xHistransferplasmid
Concentration	LotSpecific
StABIlity	1year
Storage	-20C
IntendedUse	ResearchUseOnly