Description:
pOET1C_6xHisisabaculovirustransfervectordesignedforhighlevelexpressionofforeigngenesunderthepowerfulAcMNPVpolyhedron(polh)promoter.ThevectorencodesanoptionalC-terminal6×His-Tagfusionsequencethatmaybeutilized.Thisgreatlyeasesthepurificationoftherecombinantproteinsincethe6×His-containingfusionproteinsbindwithhighaffinitytoNi-NTAAgarose.pOET1C_6xHishasaColE1originofreplicationandanampicillinresistancegeneforselectioninE.coli.Thepolhsequenceshavebeenreplacedbyamultiplecloningsite(MCS)containinguniquerestrictionsitesforinsertionoftheforeigngeneinthecorrectorientation,asshownonthecircularmap.ThecodingstrandoftheMCSastranscribedfromthepolhpromoterisshownbelowthecircularmap.TheAcMNPVsequencesflankingthegeneinthetransfervectorsMCSallowrecombinationwiththeviralDNAtoinserttheexpressioncassetteintothepolhlocus.pOET1C_6xHisiscompatIBLewithanybaculovirussystemthatutilizeshomologousrecombinationininsectcells.
AdditionalInformation:
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| Name | pOET1C6xHistransferplasmid |
|---|---|
| Concentration | LotSpecific |
| StABIlity | 1year |
| Storage | -20C |
| IntendedUse | ResearchUseOnly |