Description:

Baculovirusquantificationnowmadeeasierthanever!

Nomore plaqueassays

Nomore ELISAorimmunoassays

Nomore virusextractionspincolumns

OxfordExpressionTechnologieshasteamedupwithAgilentTechnologiestointroduceAconvenient‘allreagentsincluded’virusextraction&quantificationkit.

 

KEYFEATURES

•Titreaccuracycomparabletoplaqueassaymethod

•Lessthanonehourhands-ontime

•VirusDNAextractionandtitrationinunderthreehours

•CompatIBLewithanyAcMNPV-basedbaculovirussystemcontaininggp64viralgene

•SinglestepvirusDNAextraction:justaddlysissolution(nospincolumns)

•QPCRPrimers/Probeprovidedasasinglereagentmix:justaddwater

•Agilenthighperformance,ultrasensitivemastermixreagentincluded

•Plaqueassaytitredvirusinternalstandardsincludedforstandardcurvegeneration

 

OVERVIEWOFPROTOCOL

Seeimageforaschematicoftheprotocol

1.VirusDNAisextractedfromrecombinant virusesofunknowntitreandfromaseriesofdilutionsofthevirusinternalstandard(nocolumnsareneeded,simplyaddlysissolutiontothevirussampleandplaceintoaThermocycler)

2.QPCRiscarriedouttodeterminetheC_t valuesforthevirusDNAsamplesandinternalstandards(realtimePCRmachine)

3.ThetitresofthevirusDNAsamplesaredeterminedbyplottingtheirC_t valuesontoastandardcurvegeneratedusingvirusinternalstandards(providedinthekit)

 

KITCONTENTS

1.Lysisbuffer

2.Primer/Probemix

3.QPCRmastermix

4.Virusinternalstandard

5.DNApositivecontrol

6.RNasefreewater

7.UserGuide

Thekitcontainsreagentsadequatefor100reactions.Thisallowsforupto24titrationsintriplicateand3standardcurves. 

Pleasenotethatthekitisshippedondryice. 

AdditionalInformation:

GenWay为每个蛋白质表达项目提供定制的解决方案。我们专业的科研人员对困难的蛋白质具有丰富的经验，并已生产出2500多种重组蛋白质。无论您的需求是小型研究还是大型cGMP，我们都可以运用我们的经验来满足您的需求。服务包括大肠杆菌表达，哺乳动物表达，酵母表达和抗原制备。如果您需要报价或安排电话讨论您的需求，请使用我们方便的在线蛋白质表达报价表。

Name	baculoQUANTAll-in-onevirusextraction&titrationkit
Concentration	LotSpecific
Storage	-20C
IntendedUse	ResearchUseOnly