Description:

flashBACGOLD™providessuperiorlevelsofexpressionforanyproteinthatisdestinedforsecretionortobeinsertedinthemembrane,orforanyproteinthatmightbeparticularlyliabletodegradation.

flashBACGOLD™isabaculovirusexpressionvectorthathasbeendesignedtoreduceproteolysis,maximiseproteinsecretionandimprovemembraneproteintargetingandisbasedonourpatentedflashBAC™system,removingthenecessityforplaque-purification.ThedeletionofbothchiAandv-cathgenesfromflashBACGOLD™hasimprovedtheefficacyofthesecretorypathwayandresultedinagreatlyenhancedyieldofrecombinantproteinsthataresecretedormembranetargeted(incomparisontorecombinantvirusesthatencodechiAandv-cath).v-cathencodesacysteineprotease.Thereforedeletionofthisgeneresultsinthesignificantreductionofdegradationofprotease-sensitivetargets.Itisalsoback-compatIBLewithallexistingbaculovirustransfervectorsbasedonhomologousrecombinationininsectcellsatthepolyhedrinlocus.

AdditionalInformation:

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Name	FlashBACGold-BaculovirusExpressionSystem(96reactions)
RelatedProductNames	FlashBACGold-BaculovirusExpressionSystem(96reactions)
IntendedUse	ResearchUseOnly