Description:

Description:ThePDE5AAssayKitisdesignedforidentificationofinhibitorsofPDE5Ausingfluorescencepolarization.Phosphodiesterases(PDEs)playanimportantroleindynamicregulationofcAMPandcGMPsignaling.PDE5A,alsoknownascGMP-inhibitedphosphodiesterase,hasbeenimplicatedincardiovascularfunctionandfertility.TheassayisbasedonthebindingofafluorescentnucleotidemonophosphategeneratedbyPDE5Atothebindingagent.ThePDE5Ainhibitorscreeningassaykitcomesinaconvenient96-wellformat,withpurifiedPDE5Aenzyme,fluorescentlylabeledPDE5Asubstrate(cGMP),bindingagent,andPDEassaybufferfor100enzymereactions.ThekeytothePDE5AAssayKitisthespecificbindingagent.Usingthiskit,onlytwosimplestepsonamicrotiterplatearerequiredforPDE5Areactions.First,thefluorescentlylabeledcGMPisincubatedwithasamplecontainingPDE5Afor1hour.Second,abindingagentisaddedtothereactionmixtoproduceachangeinfluorescentpolarizationthatcanthenbemeasuredusingafluorescencereader.COMPONENTS:PDEassaybuffer:6ml;FAM-Cyclic-3?,5"-AMP:50µl@20µM;Bindingagent:100µl;Bindingagentdiluent:10ul;1blackplate,1ugPDE5A.APPLICATIONS:GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.STORAGE:-20°Cor-70°C.REFERENCES:1.MauriceDH.Front.Biosci.2005;10:1221-8.

Format:assaykit

ScientificCategory:PDE

AdditionalInformation:

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Name	PDE5AAssayKit
RelatedProductNames	PDE,phosphodiesterase,screening,inhibitors,PDE5
Format	AssayKit
Storage	Mixedstorage
IntendedUse	ResearchUseOnly