Description:

Description:TheFluorogenicHDAC5AssayKitisacompleteassaysystemdesignedtomeasurehistonedeacetylase5(HDAC5)activityforscreeningandprofilingapplications.Itcomesinaconvenient96-wellformat,withallthereagentsnecessaryfor100uluorescentHDAC5activitymeasurements.Inaddition,thekitincludespurifiedHDAC5enzymeandapotentHDACinhibitor,TrichostatinA,foruseasapositiveandnegativecontrol.TheFluorogenicHDAC5AssayKitisbasedonauniquefluorogenicsubstrateanddevelopercombination.ThisassaymethodeliminatesdealingwiththerADIoactivity,extraction,andchromatographyaspectsoftraditionalassays.Usingthiskit,onlytwosimplestepsonamicrotiterplateareneededtoanalyzetheHDAC5activitylevel.First,theHDACfluorometricsubstrate,containinganacetylatedlysinesidechain,isincubatedwithpurifiedHDAC5.ThedeacetylationsensitizesthesubstratesosubsequenttreatmentwiththeLysineDeveloperproducesafluorophorethatcanthenbemeasuredusingafluorescencereader.COMPONENTS:50005HDAC5humanrecombinantenzyme1µg-80°C50040uluorogenicHDACsubstrateclass2A(5mM)50µl-80°C500302xHDACDeveloper(containsTrichostatinA)(50µM)6ml-80°C27102TrichostatinA(200µM)100µl-20°C50031HDACassaybuffer10ul-20°Cblack,lowbindingNUNCblackmicrotiterplate1plateRoomtemp.

Format:assaykit

ScientificCategory:DeacetylaseAssay

AdditionalInformation:

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Name	HDAC5FluorogenicAssaykit
RelatedProductNames	histonedeacteylase,HDAC5
Purity	NA
Format	AssayKit
Storage	Mixedstorage
IntendedUse	ResearchUseOnly