Description:

Description:ThePDE1BAssayKitisdesignedforidentificationofPDE1Binhibitorsusingfluorescencepolarization.TheassayisbasedonthebindingofafluorescentnucleotidemonophosphategeneratedbyPDE1Btothebindingagent.PDE1Bisacalcium-dependentcyclicnucleotidephosphodiesterasethatishighlyexpressedinthestriatum.Itplaysthephysiologicalroleinthecentralnervoussystem.ThePDE1Binhibitorscreeningassaykitcomesinaconvenient96-wellformat,withpurifiedPDE1Benzyme,fluorescentlylabeledPDE1Bsubstrate(cAMP),bindingagent,andPDEassaybufferfor100enzymereactions.ThekeytothePDE1BAssayKitisthespecificbindingagent.Usingthiskit,onlytwosimplestepsonamicrotiterplatearerequiredforPDE1Breactions.First,thefluorescentlylabeledcAMPisincubatedwithasamplecontainingPDE1Bfor1h.Second,bindingagentisaddedtothereactionmixtoproduceachangeinfluorescentpolarizationthatcanthenbemeasuredusingafluorescencereader.COMPONENTS:PDEassaybuffer:6mlFAM-Cyclic-3?,5"-AMP:50µl@20µMBindingagent:100µlBindingagentdiluent:10ul1blackplate200ngPDE1Bfor100reactionsAPPLICATIONS:GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.STORAGE:-20°Cor-70°C.REFERENCES:1.SiuciakJA,McCarthySA,ChapinDS,ReedTM,VorheesCV,RepaskeDR.Neuropharmacology2007;53(1):113-24.

Format:assaykit

ScientificCategory:PDE

AdditionalInformation:

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Name	PDE1BAssayKit
RelatedProductNames	PDE,phosphodiesterase,screening,inhibitors,PDE1B
Format	AssayKit
Storage	Mixedstorage
IntendedUse	ResearchUseOnly