Description:

Description:TheHistoneAcetyltransferaseAssayKitisahomogeneous,fluorogenicassaydesignedtoscreenforinhibitorsofhistoneacetyltransferase(HAT)activityortomeasureGCN5HATactivityusingcellextractsorpurifiedenzymes.Itcomesinaconvenient96-wellformat,withallthereagentsnecessaryfor100uluorescentHATactivitymeasurements.Notime-consumingwashingstepsarerequired.Inaddition,thekitincludespurifiedGCN5(alsoknownasKAT2A)foruseasapositivecontrol.ThefluorogenicHistoneAcetyltransferaseAssayKitisbasedonthetransferofanacetylgroupfromacetylCoAtoapeptidesubstrate.AfterincubationwithacetylCoAandthesubstrate,theGCN5enzymegeneratesacetylatedH3peptideandCoASH.ThethiolgroupsofCoASHcanbedetectedwithfluorogenicreagentatexcitation=360nmandemission=460nm.Components:GCN5(KAT2A)enzyme,10?g;AcetylCoA(2.5mM),100ul;10XHATassaybuffer,5ml;52010H3peptide,500ul;FluorescenceDeveloper,100ul;DeveloperSolution,10ul;StopSolution,5ml;1black,lowbindingNUNCblackmicrotiterplate.

Format:assaykit

ScientificCategory:Acetyltransferase/Kit

AdditionalInformation:

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Name	FluorogenicGCN5AssayKit
RelatedProductNames	HAT,histoneacetyltransferase,GCN5,KAT
Format	AssayKit
Storage	MixedStorage
IntendedUse	ResearchUseOnly