Description:

Description:Aspartateaminotransferase(AST),alsoknownasGlutamate-oxaloacetatetransaminase(GOT)isatransaminase(EC2.6.1.1)similartothemoreliverspecificalaninetransaminase(ALT).Althoughcommonlyincludedclinicallyaspartofadiagnosticliverfunctiontest,ASThasabroaderclinicalutilitysinceitmayalsobeelevatedindiseasesaffectingotherorgans,suchastheheartormusclesinmyocardialinfarction,alsoinacutepancreatitis,acutehemolyticanemia,severeburns,acuterenaldisease,musculoskeletaldiseasesandtrauma.Itcatalyzesthereaction:Aspartate+?-Ketoglutarate?Oxaloacetate+GlutamateDiagnostically,itisalmostalwaysmeasuredinunits/liter(U/L).InGenWay"sASTAssayKit,anaminogroupistransferredoffromaspartateto?-ketoglutarate.TheproductsofthisreversIBLetransaminationreactionareoxaloacetateandglutamate.Theglutamateisdetectedinareactionthatconcomitantlyconvertsanearlycolorlessprobetocolor(λmax=450nm).Thekitprovidesarapid,simple,sensitiveandreliabletestsuitableasahighthroughputactivityassayofASTwithadetectionlimitof10mUperwell.
Summary:
-Detectionmethod-Absorbance(450nm)
-Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluidsetc.
-Speciesreactivity-Mammalian
-Application-ThisassaydetectsAST/SGOTwithadetectionlimitof10mUperwell.
Kitcomponents:
-ASTAssayBuffer
-ASTEnzymeMix
-Developer
-ASTSubstrate
-GlutamateStandard(0.1M)
-ASTPositiveControl
FeaturesandBenefits:
-Simpleprocedure
-Fast,reliableandconvenient
-SensitiveassaysformeasuringAST/SGOTactivityinvariousbiologicalsamplesandthekitisalsosuitableforhighthroughputanalyses.
ShippingConditions:Gelpack

AdditionalInformation:

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Name	AspartateAminotransferase(ASTorSGOT)ActivityAssayKit
RelatedProductNames	AspartateAminotransferase,AspartateAminotransferasekit,AspartateAminotransferaseassay,ASTorSGOT,ASTorSGOTkit,ASTorSGOTassay
Format	AssayKit
Storage	-20C
IntendedUse	ResearchUseOnly