Description:

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ProductName:HerpesSimplexVirus2(HSV2)IgM-ELISA

#ofSamples:1x96Assays

IntendedUse:TheHSV2IgMrecombinantELISAallowsthedetectionofHSV2infectioninpresenceofantibodiestoHSV1inhumanserumorplasma(citrate).

Introduction:HerpessimplexisanenvelopedDNAvirus(150-200nmindiameter)belongingtothealpha-herpesviridae.Basedonantigenic,biochemicalandBIOLOGicallydifferencesitcanbedividedintotwoSEROtypes,HSV-1andHSV-2.Manistheonlyknownnaturalhostandsourceofthevirus.HSVtype1typicallycausesoralherpes,whileHSVtype2typicallyaffectsthegenitalarea.Mostofthetime,HSV-1andHSV-2areinactive,or“silent”,andcausenosymptoms,butsomeinfectedpeoplehave“outbreaks”ofblistersandulcers.OnceinfectedwithHSV,peopleremaininfectedforlife.Herpessimplexvirusesareamongstthemostcommoninfectiousagentsofman,andeitherHSVtypeappearstobecapableofinfectingsimilarbodysites.Ahighpercentageoftheadultpopulation
isseropositive(appr.90%HSV-1,independenceonthesocio-economicstatus10-30%HSV-2).PrimaryHSV-1infectionusuallyoccursinearlychildhood(6to18monthsofage).HSV-2usuallyproducesmildsymptoms,andmostpeoplehavenorecognizedsymptoms.PersonsatriskarechildrenwithinheritedT-celldeficienciesandpatientswhoareimmunosuppressedbecauseofinfection(e.g.HIV),transplantation,orcancertherapy.
HSV-2cancausepotentiallyfatalinfectionsininfantsifthemotherissheddingvirusatthetimeofdelivery.-HSV-2mayplayamajorroleintheheterosexualspreadofHIV:herpescanmakepeoplemoresusceptIBLetoHIVinfection,andHIV-infectedindividualsmoreinfectious.
Thepresenceofvirusresp.infectionmaybeidentifiedby
Microscopy:CPE,IF
PCR
Serology:DetectionofantibodiesbyELISA

Principlesoftheassay:ThequalitativeimmunoenzymaticdeterminationofIgM-classantibodiesagainstHSVType2isbasedontheELISA(Enzyme-linkedImmunosorbentAssay)technique.
MicrotiterstripwellsareprecoatedwithHSVType2recombinantantigenstobindcorrespondingantibodiesofthespecimen.AfterwashingthewellstoremoveallunboundsamplematerialhorserADIshperoxidase(HRP)labelledanti-humanIgMconjugateisadded.ThisconjugatebindstothecapturedHSVType2specificantibodies.TheimmunecomplexformedbytheboundconjugateisvisualizedbyaddingTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct.TheintensityofthisproductisproportionaltotheamountofHSVType2specificIgMantibodiesinthespecimen.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorbanceat450nmisreadusinganELISAmicrowellplatereader.

StorageandStABIlity:Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.

LimitationsoftheTest:Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.Diagnosisofaninfectiousdiseaseshouldnotbeestablishedonthebasisofasingletestresult.Aprecisediagnosisshouldtakeintoconsiderationclinicalhistory,symptomatologyaswellasserologicaldata.Inimmunocompromisedpatientsandnewbornsserologicaldataonlyhaverestrictedvalue.

ReferencesCorey,L.;P.G.Spear:Infectionswithherpessimplexviruses.N.Engl.J.Med.314(1986)686-691Dörries,R.,R.Kaiser,H.Imrichetal.:NeuereAspektezurDiagnosezentralnervöserVirusinfektionen.Lab.Med.15(1991)99-102Felgenhauer,K.:DiagnostischeBedeutungderlokalsynthetisiertenspezifischenAntikörperdesLiquorcerebrospinalis.Lab.Med.15(1991)208Kaufmann,H.E.;F.MartolaandC.Dohlman:Theuseof5-iodo-2’-deoxy-uridine(IDU)inthetreatmentofherpessimplexkeratitis.Arch.Ophthal.N.Y.68(1962)235-239Spaar,F.-W.:DiemenschlicheHerpessimplexEnzephaltitsund–Meningitis.Eineklinisch-neurologischeUntersuchung.G.FischerVerlag,Stuttgart,NewYork(1976)

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Name	HerpessimplexVirus2(HSV2)IgMrecombinantantigens
RelatedProductNames	HerpesSimplexVirus2(HSV2)IgM-ELISA
MolecularWeight	0.5
Storage	Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.
IntendedUse	ResearchUseOnly