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蚂蚁淘在线 / 品牌中心 / GenWay / GenWay/TMB CORE+ Reagent/GWB-Q00248/100 ml

GenWay/TMB CORE+ Reagent/GWB-Q00248/100 ml

价格

￥4040.00

货号：GWB-Q00248

浏览量：117

品牌：GenWay

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AdditionalInformation:

GenWay为每个蛋白质表达项目提供定制的解决方案。我们专业的科研人员对困难的蛋白质具有丰富的经验，并已生产出2500多种重组蛋白质。无论您的需求是小型研究还是大型cGMP，我们都可以运用我们的经验来满足您的需求。服务包括大肠杆菌表达，哺乳动物表达，酵母表达和抗原制备。如果您需要报价或安排电话讨论您的需求，请使用我们方便的在线蛋白质表达报价表。

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/**/

Name	TMBCORE+Reagent
RelatedProductNames	TMBCORE+Reagents,ELISASolutions,TMBCORE+
ProductFormat	ReadytouseTMBsolution-liquid
Applications	ELISA
ApplicationInfo	ELISA:Neat
Format	ReadyToUse
StABIlity	48monthsfromdateofdespatch.
ReconstitutionandStorage	Storeat+4^oC.DONOTFREEZE. Thisproductisphotosensitiveandshouldbeprotectedfromlight. Avoidexposuretoheatandcontaminationwithmetalionsorperoxidase. StoreinbottlesmadeofHighDensityPolyethylene(HDPE). ShelfLife 48monthsfromdateofdespatch.
Storage	Storeat+4^oC.DONOTFREEZE. Thisproductisphotosensitiveandshouldbeprotectedfromlight. Avoidexposuretoheatandcontaminationwithmetalionsorperoxidase. StoreinbottlesmadeofHighDensityPolyethy
LeadTime	Domestic:within2-3weeksdelivery International:2-3weeks
IntendedUse	ResearchUseOnly
::	IntendedUse:ThisproductisahighperformanceTMB(3,3",5,5"-tetramethylbenzidine)solution,recommendedforuseinELISAasasubstrateforhorserADIshperoxidase(HRP). ThisproductcontainsTMB,substratebufferandhydrogenperoxideinasafe,readytousesolution.TheactivityofTMBhasbeenoptimisedtoenableincreasedsensitivity,minimalbackgroundandrapiddevelopment. ThisproductproducesadeepbluecolourduringtheenzymaticdegradationofH₂O₂byhorseradishPeroxidase.Thereactionmaybestoppedwith0.2Msulphuricacid,resultinginayellowcolourreadat450nm. TMBPLUS2hassimilarsensitivitytoTMBPLUS(GWB-Q00251orGWB-Q00252),butanincreasedshelflife.
::	InstructionsForUse: 1.Itisrecommendedthat100ulofGWB-Q00248TMBsubstrateisusedpermicrotiterwell.Pourthedesiredamountofsubstrateintoasealedcontainerandallowittoreachroomtermperatureinthedark. 2.Add100ulsubstratesolutionpermicrotiterwell. 3.Allowdevelopmentofthesubstratesolution.Timeofdevelopmentistypically5-30minutes.Forbestresults,theplateshouldbekeptinthedarkduringincubatione.g.wrappedintinfoil. 4.Forkineticassays,readabsorbanceat655nm(blue).Forendpointassays,addanequalvolumeof0.2Msulphuricacidandreadtheabsorbanceat450nm(yellow).Thisendpointsolutionisstableforuptoonehour. N.B.Ifreducedintensityisrequired,itisrecommendedthatthedevelopmenttimeisreducedortheantibody/conjugateisdilutedfurther.(DilutionofGWB-Q00248isnotrecommended).