Description:

Description:ADIpogenesisistheprocessofdifferentiationofdifferentcelltypesintoadipocytes,theprimaryfatstoragecelltype.Theaccumulationofadipocytesisthebasisforobesity,asignificantriskfactorinmanydiseases,includingdiabetes,atherosclerosis,cancerandcardiovasculardisease,etc.Adipocytesaccumulatetriglycerides,intheformoflipiddropletswhichcanbemeasured.GenWay"sadipogenesisassaykitquantifiestriglycerideaccumulationincellsandtissues.Intheassay,triglyceridesareefficientlysolubilizedthenhydrolyzedtoglycerolwhichissubsequentlyoxidizedtoconverttheprobetogeneratecolor(λmax=570nm)andfluorescence(Ex/Em=535/587nm).Thekitcandetecttriglycerideinasfewasathousandorlessdifferentiated3T3-L1cellswithtriglyceridedetectionlinearrange0.2to10nmol.Thehighdetectionsensitivityandtheconvenientmicroplateassayformatmakethekitaconvenienttoolforstudyingtheeffectofadipogenesisinducersorinhibitors,ortoscreendrugs.
Summary:
-Detectionmethod-Absorbance(570nm)orFluorescence(Ex/Em535/587nm)
-Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluids
-Speciesreactivity-Mammalian
-Application-Thekitcandetecttriglycerideinasfewasathousandorlessdifferentiated3T3-L1cellswithtriglyceridedetectionlinearrange0.2to10nmol.
Kitcomponents:
-AdipogenesisAssayBuffer
-LipidExtractionSolution
-AdipogenesisProbe(inDMSOsolution)
-Lipase(lyophilized)
-AdipogenesisEnzymeMix(lyophilized)
-TriglycerideStandard(1mM)
FeaturesandBenefits:
-Simpleprocedure;takes~40minutes
-Fastandconvenient
-Kitcontainsallnecessaryreagentsforrapid,sensitiveandaccuratemeasurementofAdipogenesisinvarioussamples.
ShippingConditions:Gelpack

AdditionalInformation:

GenWay为每个蛋白质表达项目提供定制的解决方案。我们专业的科研人员对困难的蛋白质具有丰富的经验，并已生产出2500多种重组蛋白质。无论您的需求是小型研究还是大型cGMP，我们都可以运用我们的经验来满足您的需求。服务包括大肠杆菌表达，哺乳动物表达，酵母表达和抗原制备。如果您需要报价或安排电话讨论您的需求，请使用我们方便的在线蛋白质表达报价表。

Name	AdipogenesisAssayKit
RelatedProductNames	Adipogenesis,Adipogenesisassay,Adipogenesiskit,Adipogenesisassaykit,Adipogenesisassays,Adipogenesisdetermination,Adipogenesisanalysis,Adipogenesisdetection,Adipogenesismeasurement,Adipogenesisquantification
Format	AssayKit
Storage	-20C
IntendedUse	ResearchUseOnly