General Information

Product

TdT Antibody

Description

Affinity Purified DNA nucleotidylexotransferase Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 450-500 on human Terminal deoxynucleotidyltransferase protein.

Accession #

NCBI: NP_004079

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:1,000.Detects a band of approximately 58 kDa.

Protein

Uniprot #

P04053

Overview

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5-triphosphate to the 3-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

Molecular Function

Nucleotidyltransferase, Transferase, DNA binding

Subcellular Location

Nucleus

Structure

Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA.

Alternative Nomenclature

Deoxynucleotidyltransferase terminal antibodyDNA nucleotidylexotransferase antibodyDNTT antibodyNucleosidetriphosphate DNA deoxynucleotidylexotransferase antibodyTDT antibody Terminal addition enzyme antibodyTerminal deoxynucleotidyltransferase antibodyTerminal transferase antibody