General Information

Product

Myocilin Antibody

Verified Applications

ELISA, ICC/IF, IHC, IP, Immunomicroscopy,WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide corresponding to N terminal amino acids 25-46 of Myocilin. Peptide was covalently modified and conjugated to KLH.

Sequence:

CLWDVGARTAQLRKANDQSGR

Accession #

GenBank: BAA24532.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500. Predicted molecular weight:55 kDa.

Protein

Uniprot #

Q99972

Overview

Secreted glycoprotein regulating the activation of different signaling pathways in adjacent cells to control different processes including cell adhesion, cell-matrix adhesion, cytoskeleton organization and cell migration. Promotes substrate adhesion, spreading and formation of focal contacts. Negatively regulates cell-matrix adhesion and stress fiber assembly through Rho protein signal transduction.

Molecular Function

fibronectin binding, myosin light chain binding, frizzled binding

Subcellular Location

Secreted, Golgi apparatus, Cytoplasmic vessicle

Expression

Expressed in large amounts in various types of muscle, ciliary body, papillary sphincter, skeletal muscle, heart, bone marrow-derived mesenchymal stem cells and other tissues. Expressed predominantly in the retina. In normal eyes, found in the inner uveal meshwork region and the anterior portion of the meshwork. In contrast, in many glaucomatous eyes, it is found in more regions of the meshwork and appeared more intensively than in normal eyes, regardless of the type or clinical severity of glaucoma.

Structure

Homodimer (via N-terminus). Interacts with OLFM3, FN1, NRCAM, GLDN and NFASC. Interacts (via N-terminus) with MYL2. Interacts with SFRP1, FRZB, FZD7, FZD10, FZD1 and WIF1; regulates Wnt signaling. Interacts with SNTA1; regulates muscle hypertrophy. Interacts with ERBB2 and ERBb3; activates ERBB2-ERBB3 signaling pathway. Interacts with SNCG; affects its secretion and aggregation.

Alternative Nomenclature

Glaucoma 1 open angle antibody

GLC1A antibody

GPOA antibody

JOAG antibody

Mutated trabecular meshwork-induced glucocorticoid response protein antibody

MYOC antibody

Myocilin antibody

TIGR antibody