Product | PDE4A Antibody |
Description | Affinity Purified Phosphodiesterase 4A Antibody |
Verified Applications | CM, ELISA, ICC, IF, IHC, IP, WB |
Host | Rabbit |
Species Cross Reactivity | Human, Moose, Mouse, Rat |
Immunogen | Synthetic cyclic peptide corresponding to the C-terminal region of PDE4A. Common to all PDE4A proteins. |
| Specificity | This antibody labels all known PDE4A variants including PDE4A1 (66 kDa), PDE4A5 (109 kDa), PDE4A8 (106 kDa), AND PDE4Ax (10 kDa) and a 76 kDa testis specific A variant. |
Physical Properties | |
Quantity | 100 µg |
Volume | 200 µl |
Concentration | 0.5 µg/µl in antibody stabilization buffer |
Form | Affinity Purified |
Clonality | Polyclonal |
Storage | -20⁰C for long term storage |
Application Protocol | |
Confocal Microscopy | 1:100-1:400 |
DB | 1:10,000 |
ELISA | 1:10,000 |
Immunocytochemistry | 1:100-1:400 |
Immunofluorescence | 1:100-1:400 |
Immunohistochemistry | 1:100-1:400 |
Immunoprecipitation | 1:250 |
Western Blot | 1:1,000-1:2,500 |
Protein | |
Uniprot # | P27815 |
Overview | Hydrolyzes the second messenger cAMP which is a key regulator of many important physiological processes. |
Molecular Function | cAMP |
Subcellular Location | Isoform 1: perinuclear region, Isoform 2: ruffle membrane, Isoform 4: Peripheral membrane protein, Isoform 6: perinuclear region, Isoform 7: Membrane, Cytoplasm |
Expression | Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lympohocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level). |
Structure | Belongs to cyclic nucleotide phosphodiesterase family. PDE4 subfamily |
Alternative Nomenclature | 5-cyclic phosphodiesterase 4A cAMP specific 3 5 cyclic phosphodiesterase 4A DPDE2 dunce like phosphodiesterase E2 PDE 4A PDE4 PDE46 PDE4A11 Phosphodiesterase 4A phosphodiesterase 4A cAMP specific phosphodiesterase E2 dunce homolog phosphodiesterase isozyme 4 phosphodiesterase type 4A11 |
Protein information supplied by UniProt
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