Product | ULK3 Antibody |
Description | Affinity Purified Serine/Threonine-protein kinase ULK3 Antibody C-epitope |
Verified Applications | ELISA, IHC, IP,WB |
Host | Rabbit |
Species Cross Reactivity | Bovine, Human, Monkey, Mouse, Rat |
Immunogen | Synthetic peptide corresponding to a region within C terminal amino acids 452-472 of Mouse ULK3, coupled to a carrier protein. |
Accession # | NCBI: NP_001258064.1 |
Physical Properties | |
Quantity | 100 µg |
Volume | 200 µl |
Concentration | 0.65 µg/µl in antibody stabilization buffer |
Form | Affinity Purified |
Clonality | Polyclonal |
Determinant | C-epitope |
Storage | -20⁰C for long term storage |
Application Protocol | |
DB | 1:10,000 |
ELISA | 1:10,000 |
| Immunohistochemistry | 1:50-1:100 |
Immunoprecipitation | 1:200 |
Western Blot | 1:500 |
Protein | |
Uniprot # | D3ZHP7 |
Overview | Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy. |
Molecular Function | ATP-binding; Nucleotide-binding; Serine/threonine-protein kinase |
Subcellular Location | Cytoplasm |
Expression | Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord. |
Structure | Interacts (via protein kinase domain) with SUFU. |
Alternative Nomenclature | DKFZP434C131 antibody FLJ90566 antibody Serine/threonine-protein kinase ULK3 antibody Ulk3 antibody unc 51 like kinase 3 (C. elegans) antibody Unc-51-like kinase 3 antibody |
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