FabGennix蚂蚁淘在线

商品

品牌

文章

热门搜索：

快报

蚂蚁淘特色产品FLOWTECH造影剂蚂蚁淘授权代理品牌-德国Emfret 蚂蚁淘旗下188精品现货平台正式上线啦！！！蚂蚁淘四月促销蚂蚁淘获得vitanavitech代理商授权蚂蚁淘获得QA-bio代理商授权蚂蚁淘获得Viagenbiotech代理商授权 polysciences公司24765-1到现货了(内附代理商整理说明书）蚂蚁淘四月团建“环苏州古城一周” Clodronate Liposomes用量更正！

品牌首页品牌新闻品牌简介产品中心品牌咨询联系我们

品牌咨询

联系方式

公司地址

苏州工业园区生物纳米园A4#216

联系电话

4000-520-616 / 18915418616

传真号码

0512-67156496

电子邮箱

info@ebiomall.com

公司网址

https://www.ebiomall.com

蚂蚁淘在线 / 品牌中心 / FabGennix / FabGennix/Phospho-MERTK Antibody/PMKT-140AP

FabGennix/Phospho-MERTK Antibody/PMKT-140AP

价格

￥7000.00

货号：PMKT-140AP

浏览量：127

品牌：FabGennix

服务

全国联保

正品保证

正规发票

签订合同

加入收藏

立即购买

商品描述

General Information
Product	Phospho-MERTK Antibody
Description	Affinity Purified Phosphorylated Tyrosine-protein kinase Mer Antibody
Verified Applications	CM, ELISA, FC, ICC, IF, IHC, WB
Host	Rabbit
Species Cross Reactivity	Human, Monkey, Mouse, Rat
Immunogen	Synthetic peptide corresponding to amino acids 746-757 ofHuman MERTK. Sequence: CKKIY_pSGDY_pY_pRQG
Accession #	NCBI: NP_075232.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.63 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:250
DB	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:250
Immunofluorescence	1:250
Immunohistochemistry	1:250
Western Blot	1:750. Observed band of 180kDa
Protein
Uniprot #	P57097
Overview	Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules.
Molecular Function	Kinase, Receptor, Transferase, Tyrosine-protein kinase
Subcellular Location	Membrane; Single-pass type I membrane protein
Structure	Interacts (upon activation) with TNK2; stimulates TNK2 autophosphorylation. Interacts (via N-terminus) with extracellular ligands LGALS3, TUB, TULP1 and GAS6. Interacts with VAV1 in a phosphotyrosine-independent manner.
Alternative Nomenclature	c MER antibody c mer proto oncogene tyrosine kinase Eyk MER MERPEN Mertk MERTK c-mer proto-oncogene tyrosine kinase nmf12 Nyk Proto oncogene tyrosine protein kinase MER Receptor tyrosine kinase MerTK RP38 STK kinase Tyrosine-protein kinase Mer

Protein information supplied by UniProt

FabGennix Phospho-MerTK Antibody has been used in 26Publications

Applications: Confocal microscopy, Flow cytometry, Immunocytochemistry, Immunohistochemistry Immunofluorescence, and Western blot

Reactivity: Human, Mouse, and Rat

ZCai, Bishuang, et al. "MerTK signaling in macrophages promotes the synthesis of inflammation resolution mediators by suppressing CaMKII activity." Sci. Signal. 11.549 (2018).

PMID:30254055

Applications:Western Blot

Reactivity: Human

Zhang, Bing, et al. "Mer receptor tyrosine kinase negatively regulates lipoteichoic acid-induced inflammatory response via PI3K/Akt and SOCS3." Molecular immunology 76 (2016).

PMID: 27419619

Applications: Western Blot

Reactivity: Mouse

Das, Amitava, et al. "Correction of MFG-E8 resolves inflammation and promotes cutaneous wound healing in diabetes." The Journal of Immunology 196.12 (2016).

PMID: 27194784

Applications: Western Blot

Reactivity: Mouse

Cai, Bishuang, et al. "MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation." Proceedings of the National Academy of Sciences 113.23 (2016).

PMID: 27199481

Applications: Western Blot

Reactivity: Mouse

Ginisty, Aurélie, et al. "Evidence for a Subventricular Zone Neural Stem Cell Phagocytic Activity Stimulated by the Vitamin K‐Dependent Factor Protein S." STEM CELLS 33.2 (2015).

PMID: 25308179

Applications: Western Blot

Reactivity: Rat

Nguyen, Khanh-Quynh N., et al. "Overexpression of MERTK receptor tyrosine kinase in epithelial cancer cells drives efferocytosis in a gain-of-function capacity." Journal of Biological Chemistry 289.37 (2014).

PMID: 25074939

Applications: Western Blot

Reactivity: Human

Law, Ah-Lai, et al. "Cleavage of Mer Tyrosine Kinase (MerTK) from the Cell Surface Contributes to the Regulation of Retinal Phagocytosis." Journal of Biological Chemistry (2014).

PMID: 25538233

Applications: Western Blot

Reactivity: Mouse

Suleiman, Lutfi, Claude Négrier, and Habib Boukerche. "Protein S: A multifunctional anticoagulant vitamin K-dependent protein at the crossroads of coagulation, inflammation, angiogenesis, and cancer."Critical Reviews in oncology/hematology88.3 (2013).

PMID: 23065156

Applications: Western Blot

Reactivity: Mouse

Wang, Y, et al. "Mer Receptor Tyrosine Kinase Promotes Invasion and Survival In Glioblastoma Multiforme."Oncogene32.7 (2013): 872-882.

PMID: 22469987

Applications: Confocal Microscopy, Immunofluorescence, Immunohistochemistry, Western Blot

Reactivity: Human

Choi, Ji-Yeon, et al. "Upregulation of Mer receptor tyrosine kinase signaling attenuated lipopolysaccharide-induced lung inflammation." Journal of Pharmacology and Experimental Therapeutics 344.2 (2013).

PMID: 23197771

Applications: Western Blot

Reactivity: Mouse

Yefimova, Marina G. et al. “A Chimerical Phagocytosis Model Reveals the Recruitment by Sertoli Cells of Autophagy for the Degradation of Ingested Illegitimate Substrates.” Autophagy9.5 (2013).

PMCID: 3669177

Applications: Confocal Microscopy, Immunocytochemistry, Immunofluorescence, Western Blot

Reactivity: Human

Click Here for the complete list of all FabGennix Phospho-MerTK Antibody publications

Publishing research using PMKT-140AP? Please let us know, we can cite the reference in this datasheet.

To view the Specification Sheet for Phospho-MerTKAntibody, please click the link below:

Phospho-MerTK Antibody| FabGennix

I run cell lysates from different human cell lines corresponding to 50 ug total protein (lysis buffer 25 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, 10% glycerol, 1% Triton X-100 with protease and phosphatase inhibitors) under reducing conditions on a 8% gel followed by blotting to a PVDF membrane. As blocking reagent I used 5% skim Milk in 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20, 1 h RT. Followed by 3 washes with TBST buffer (50 mM Tris pH 8, 150 mM NaCl, 0.1% Tween-20).

I incubated the blot with the above mentioned antibody for overnight at 4 C on rotating shaker diluted 1:750 in BSA buffer (5% BSA in 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20 ).

Next day, followed by 6 washes with 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20. As a secondary antibody I used a anti-rabbit HRP conjugate from cell signaling, diluted 1:10000 in blocking buffer. Incubation time again 1 h RT. The membrane was washed 6 times as above and developed using ECL.

After phosphoMer detection, the blot was washed 6 times with 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20 was analysed for total MERTK using the AF891 from R & D system. Similar process was used for total MERTK also.

Results-

When detecting phosphoMer I see several bands from 130 KD to 250 KD. whereas in total MERTK I see a single band.

We know now for sure that:

phospho-MER protein showed some abnormality in the migration on SDS-PAGE, so therefore the proteins will not be exactly at the same place in phospho-lane.

The non-phospho-MER has differences in the MW due to differential glycosylation on the core protein. This was very elegantly published by Doug Graham (who cloned this gene) using some of our reagents

I am not sure about the characteristics of non-phospho-MER antibodiesused in these experiments. Do they recognize all forms of differentially modified MER or just recognize the core proteins and does not recognize the fully mature MER proteins in the samples?

Our non-Phospho-MER antibodies (MKT-101AP, MKT-112AP and MKT-121AP) recognize not only the core proteins but also the glycosylated from different tissues and cells extracts.

In some samples our Phospho-mer antibody (PMKT-140AP) also recognize a smaller (just above 90kDa) band. The MW of these proteins are apparent in relation to the MW standards and cannot be considered as actual size of the proteins. These numbers will vary quite significantly on different pore size gels, and sometimes even when different companies MW markers are used. We also know that calculated MW will also vary when colored and non-color MW makers are compared on the same gel.

I run cell lysates from different human cell lines corresponding to 50 ug total protein (lysis buffer 25 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, 10% glycerol, 1% Triton X-100 with protease and phosphatase inhibitors) under reducing conditions on a 8% gel followed by blotting to a PVDF membrane. As blocking reagent I used 5% skim Milk in 50 mM Tris pH 8, 150 mM NaCl, 0.5% Tween-20, 1 h RT. Followed by 3 washes with TBST buffer (50 mM Tris pH 8, 150 mM NaCl, 0.1% Tween-20).

Results-

When detecting phosphoMer I see several bands from 130 KD to 250 KD. whereas in total MERTK I see a single band.

We know now for sure that:

phospho-MER protein showed some abnormality in the migration on SDS-PAGE, so therefore the proteins will not be exactly at the same place in phospho-lane.

The non-phospho-MER has differences in the MW due to differential glycosylation on the core protein. This was very elegantly published by Doug Graham (who cloned this gene) using some of our reagents

Our non-Phospho-MER antibodies (MKT-101AP, MKT-112AP and MKT-121AP) recognize not only the core proteins but also the glycosylated from different tissues and cells extracts.

Accessories

Product

Note

Status

Price

Phospho-MERTK Antibody FITC

PMKT-FITC

$395.00*

Phospho-MERTK Antibody BIOTIN

PMKT-BIOTIN

$395.00*

Phospho-MERTK Blocking Peptide

P-PMKT

$180.00*

Phospho-MERTK Positive Control

PC-PMKT

$205.00*

Shipping Options

Bulk and Special Orders Quote

Display accessory details

We also recommend

MKT-121AP MERTK Antibody $295.00*	MKT-101AP MERTK Antibody $295.00*	MKT-112AP MERTK Antibody $295.00*
TYRO3-101AP TYRO3 Antibody $295.00*

Shipping Options

Bulk and Special Orders Quote

Customers who bought this product also bought

MERTK Antibody

$295.00*

Phospho-MERTK Positive Control

$205.00*

Shipping Options

Bulk and Special Orders Quote

Browse these categories as well:Neurotransmission, Tyrosine Kinases, Vision, Primaries, Receptor Tyrosine Kinases

Fabgennix 品牌介绍官网：http://www.fabgennix.com/ Fabgennix公司是一家知名的抗体研发生产企业，为全球生物技术公司、科研单位和生物制药公司提供一抗、二抗、试剂、试剂盒、多肽和阳性质控品等产品。基于坚实的科研背景，Fabgennix公司已成为全球免疫诊断市场高质量单克隆抗体的优质供应商。 Fabgennix 主要产品分类【一抗】 1. 癌症研究 2. 免疫学 3. 微生物学 4. 信号转导 5. 新陈代谢和内稳态 6. 视力和嗅觉 7. 通道和转运蛋白 8. 表观遗传学 9. 干细胞 10. 细胞生物学 11. 神经病学 12. 心脏病学 13. 内分泌系统【二抗】 1. Rabbit Anti-Chicken IgG HRP 【试剂】 1. Western Blot Stripping Buffer 2. 硝酸纤维膜蛋白染料【试剂盒】 1. 细胞溶解试剂盒 2. PDEase 试剂盒 3. 外周血单核细胞（PBMC）分离试剂盒【实验室服务】 1. 客户抗体显影 2. 蛋白化学多肽合成实验室 3. 免疫学实验室 4. 影像处理实验室 5. 免疫组化实验室

资质认证

获得国家资质，权威认证！
全国联保

全国联保，官方无忧售后
正规发票

正规发票，放心购买
签订合同

签订合同，保障您的权益

/**/