GFP-Trap^®_M

Effective pulldown for consistent results

Description:anti-GFP V_HH coupled to magnetic microparticles for immunoprecipitation of GFP-fusion proteins

Specificity:

• AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K
• CFP, eCFP, mCerulean
• YFP, Citrine, eCitrine, eYFP, Venus, Ypet
• BFP

Applications:

• Immunoprecipitation/ Co-IP
• Mass spectrometry
• Enzyme activity measurements
• RIP analysis

Bead properties:Bead size: ~ 0.5 -1µm magnetic microparticles (Chemicell)Storage buffer: 1x PBS, 0.05% sodium azideBinding capacity: 10µl GFP-Trap^®_M slurry binds 0.25 – 0.5µg of recombinant GFP

Storage instructions:Shipped at ambient temperature. Upon receipt store at 4°C; stable for 6 months.

Effective pulldown of GFP-fusion proteins for consistent results

• No heavy & light antibody chains, short incubation (5-30 min)

• Extraordinary binding, also under harsh conditions

• Very high affinity (KD=1pM*) to bind even low abundant proteins

• Reliable gold standard with more than 900 publications

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