Description Purified recombinant Enhanced Green Fluorescent Protein (EGFP); full length protein; fused to 6xHis-tag
Applications
- Immunoprecipitation: use ~5 µg EGFP per 25 µL GFP-Trap® bead slurry
- SDS-PAGE: use ~ 1 µg EGFP per lane
- Western blot: use ~25 ng - 100 ng EGFP per lane
- ELISA: coat ~ 1ng - 50 ng per well of a 96-well plate
- General: as a fluorescent standard and for calibration