ASPP1 (Apoptosis-stimulating of p53 protein 1, Protein phosphatase 1 regulatory subunit 13B, PPP1R13B) plays a central role in regulating apoptosis via its interaction with p53. ASPP1 acts on p53 by enhancing the DNA binding and transactivation function of p53 on the promoters of proapoptotic genes in vivo. The expression of ASPP1 is frequently downregulated in human breast carcinomas expressing wild-type p53 but not mutant p53. Abbiotec提供了293个细胞的10,000多种裂解物，这些细胞被编码人类基因组25,000种蛋白质之一的质粒转染。裂解液是由用TrueORF基因携带的pCMV质粒（OriGene Technologies，Inc.）瞬时转染的HEK293T细胞制备的，然后在RIPA缓冲液中裂解。人全长蛋白带有C端Myc / DDK标签以进行检测。TACL™产品系列在Western印迹和免疫沉淀，ELISA标准品和反向蛋白质阵列中用作阳性对照。每种产品均包含纯载体转染裂解物作为阴性对照。