Product Information

The BIP-300 plasmid allows you to compare your pAN or pAC AviTagTM fusion gene"s protein expression against a confirmed AviTag^TM fusion product under the same growth and induction conditions. When transformed into the same E. coli host strain as your own pAN or pAC AviTagTM fusion plasmid construct, and induced in parallel, the BIP-300 Positive Control Plasmid will provide a measure by which to compare your transformation efficiency, growth and antibiotic maintenance conditions, and induction of your plasmid"s gene-fusion construct. The BIP-300 construct consists of a modified β-galactosidase gene (8 amino acids were deleted from its N-terminus) cloned into Avidity"s pAN5 AviTag^TM fusion vector at the Multiple Cloning Site. The resulting AviTag^TM-protein fusion (AviTag-β-gal), as are all the pAN and pAC fusion products, is under the tight control of the Trc promoter system and expressed upon IPTG induction. The plasmid is maintained with ampicillin. An example induction protocol is given under Protocols. This protocol uses the Avidity AVB101 E. coli strain containing the BirA over-expressing pACYC-184 plasmid construct (pBirAcm) as the host strain. It is initially requires chloramphenicol for maintenance in addition to the ampicillin required to maintain the pAN or pAC plasmid construct. If you are using a different host strain please adjust the protocol accordingly.

Supplied as 1 vial of Positive Control Plasmid DNA; 2µg at 1mg/mL in nuclease-free water (2µL). Store at -20°C. Avidity研发和销售用于分子连接的分子亲和工具，其的AviTag™技术采用针对性很强的一个独特的15氨基酸肽，用来自大肠杆菌的生物素连接酶（BirA）标签上的单一的生物素酶的共轭。   Avidity develops and sells molecular affinity tools for connecting molecules. Our patented AviTag™ technology employs a highly targeted enzymatic conjugation of a single biotin on a unique 15 amino acid peptide tag using the biotin ligase （BirA） from E. coli. AviTag™ sequence is GLNDIFEAQKIEWHE.  Oriented on streptavidin-coated surfaces, this creates an ideal presentation for molecular binding interactions. 公司：www.avidity.com   产品编号 产品名称 规格 价格（元） 生产 AbC Anti-C-terminus AviTagTM Antibody 100 ul ￥3,600.00 Avidity AbCA Anti-C-terminus AviTagTM –conjugated Agarose Resin 2 ml ￥4,500.00 Avidity AVB100 Efficient Biotinylation E. coli K12 Strain AVB100 1 vial ￥4,500.00 Avidity AVB101 Efficient Biotinylation E. coli B Strain AVB101 1 vial ￥4,500.00 Avidity AVB99 Efficient Biotinylation E.coli K12 Strain AVB99 1 vial ￥4,500.00 Avidity BIO200 Biotin Solution 1.5 ml ￥810.00 Avidity BIO500 Biotin Solution 10 ml ￥1,620.00 Avidity BIP300 Positive Control Plasmid 2 ug ￥2,700.00 Avidity BIRA500 BirA Biotin-Protein Ligase 40 ug ￥4,500.00 Avidity BIS300 Positive Control Protein Substrate Kit 1 kit ￥2,700.00 Avidity Bulk BIRA Bulk BirA Biotin-Protein Ligase 300 ug ￥17,640.00 Avidity CFT100 Mammalian Cell-Free Translation System: AvidExpressTM 1 kit ￥3,150.00 Avidity CTZ-1 Native Coelenterazine Free Base 1 mg ￥3,600.00 Avidity CVB101 Chemically Competent E.coli B Strain AVB101 10 vials ￥3,150.00 Avidity EVB100 Electrocompetent E. coli K12 Strain AVB100 10 vials ￥3,150.00 Avidity EVB101 Electrocompetent E. coli B Strain AVB101 10 vials ￥3,150.00 Avidity FASP Sequence Primer-Forward 200 pmol ￥1,710.00 Avidity Gluc-10 Biotinylated Gaussia princeps Luciferase 10 ug ￥1,350.00 Avidity Gluc-100 Biotinylated Gaussia princeps Luciferase 100 ug ￥6,300.00 Avidity pAC4 Biotin AviTagTM Vectors（C-terminal） 10 ug ￥2,970.00 Avidity pAC5 Biotin AviTagTM Vectors（C-terminal） 10 ug ￥2,970.00 Avidity pAC6 Biotin AviTagTM Vectors（C-terminal） 10 ug ￥2,970.00 Avidity pACS Biotin AviTagTM Vectors（C-terminal） 1 kit ￥8,100.00 Avidity pAN4 Biotin AviTagTM Vectors（N-terminal） 10 ug ￥2,970.00 Avidity pAN5 Biotin AviTagTM Vectors（N-terminal） 10 ug ￥2,970.00 Avidity pAN6 Biotin AviTagTM Vectors（N-terminal） 10 ug ￥2,970.00 Avidity pANS Biotin AviTagTM Vectors（N-terminal） 1 kit ￥8,100.00 Avidity RASP Sequence Primer-Reverse 200 pmol ￥1,710.00 Avidity rrGFP-100 Biotinylated Renilla reniformis Green Fluorescent Protein 100 ug ￥5,400.00 Avidity SA-Gluc Streptavidin-conjugated Gaussia princeps Luciferase 50 ug ￥4,500.00 Avidity SA-rrGFP 1.0 Streptavidin-conjugated Renilla reniformis Green Fluorescent Protein 160 ug ￥3,600.00 Avidity