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RecombinantbioluminescentphotoproteinfromObelialongissima.Thephotoproteinisapproximately21,000molecularweightandcontainstheluminescentsubstrate,nativecoelenterazine,andoxygenboundtotheprotein.Uponadditionofexcesscalciumions,thatfillthethreecalcium-bindingsites,theoxygenreactswiththesubstratetoyieldarapidflashofbluelightcenteredat485nm.Thisemissionreachesapeakinlessthan100msec.anddecaysinlessthan1second.