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蚂蚁淘在线 / 品牌中心 / arp1 / American Research Products/AAV-TRE-CAS9/Vector + Lentiviral Packaging Kit/CAS9-16-Vector + Lentiviral Packaging Kit
商品描述
ProductName | AAV-TRE-CAS9 |
---|---|
Description | ThevectorAAV-TRE-Cas9isdesignedforexpressionofCas9nucleaseundertheTREpromotercontrol,andthevectorAAV-CMV-EGFP--P2A-tTA3g-U6sgRNAisdesignedforexpressionofsingle-guideRNAandtTA3gwhichwillbindtotheTREpromotertopromotetheexpressionofCas9.Auser-definedsgRNAiscloneddownstreamofahumanU6promoter.ToconstructtheAAV-CMV-EGFP--P2A-tTA3g-U6sgRNAvector,youmustconstructpGL3-U6sgRNA-Purowithyourguidesequence.TheU6-gRMAsequenceisobtainedbyPCR,andthePCRproductionisclonedintothepre-linearizedvectorusingtheincludedhigh-efficiencyligationmixandcompetentcells.InthepresenceoftheCas9endonuclease,sgRNAcanrecognizethetargetedDNAsequenceandguideCas9nucleaseduringgenomeeditingforgeneknockout,knockin,mutagenesis,andmore. |
PackageContents | 20µlAAV-TRE-Cas9Vector(7.5ng/µl) |
PlasmidIdentity | -Vectoridentitywasconfirmedbysequencing.-A260/A280:1.8-2.0 |
Storage | Storeat-20°C.Avoidrepeatedfreeze/thawcycles.ShippedwithIceGelPacks. |
StorageBuffer | 10mMTris-HCl(pH8.0),1mMEDTA(pH8.0) |