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蚂蚁淘在线 / 品牌中心 / arp1 / American Research Products/LENTI-EF1A-CAS9-P2A-PURO/Plasmid Only/CAS9-05-Plasmid Only
商品描述
ProductName | LENTI-EF1A-CAS9-P2A-PURO |
---|---|
Description | Thevectorlenti-EF1a-Cas9-P2A-PuroisdesignedforexpressionofCas9nucleaseformammaliangenemodificationstudiesusingCRISPR/Cas9technologyinvivoorinvitro.Cas9isexpressedfromabidirectionalEF1apromoter.TheCas9nucleaseisfusedwithNuclearLocalizationSignal(NLS)andflagtagintheNterminusunderthecontrolofEF1apromoter,nuclearlocalizationsignals(NLS)attheN-terminusistoensureefficientimportofthehspCas9proteinintothenucleus,andflag-tagisusedfordetectionandpurificationoftherecombinantCas9protein.TheCas9nucleaseisfollowedbyaregulatoryelementcalledWPRE(Woodchuckviruspost-transcriptionalregulatoryelement)toboostgeneexpressionandstABIlizethemRNAtranscript. |
PackageContents | 20µllenti-EF1a-Cas9-P2A-PuroVector(7.5ng/µl) |
PlasmidIdentity | -Vectoridentitywasconfirmedbysequencing.-A260/A280:1.8-2.0 |
Storage | Storeat-20°C.Avoidrepeatedfreeze/thawcycles.ShippedwithIceGelPacks. |
StorageBuffer | 10mMTris-HCl(pH8.0),1mMEDTA(pH8.0) |