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蚂蚁淘在线 / 品牌中心 / arp1 / American Research Products/LENTI-EF1A-CAS9-EGFP-U6 SGRNA/Plasmid Only/CAS9-18-Plasmid Only
商品描述
ProductName | LENTI-EF1A-CAS9-EGFP-U6SGRNA |
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Description | Thevectorlenti-EF1a-Cas9-EGFP-U6sgRNAisdesignedforexpressionofCas9andgRNAformammaliangenemodificationstudiesusingCRISPR/Cas9technologyinvivoorinvitro.Cas9isexpressedfromabidirectionalEF1apromoterandsgRNAiscloneddownstreamofahumanU6promote.Toconstructthevector,youmustconstructpGL3-U6sgRNA-Purowithyourguidesequence.TheU6-gRMAsequenceisobtainedbyPCR,andthePCRproductionisclonedintothepre-linearizedvectorusingtheincludedhigh-efficiencyligationmixandcompetentcells.Afterinjectedwiththevector,sgRNAcanrecognizethetargetedDNAsequenceandguideCas9nucleaseduringgenomeeditingforgeneknockout,knockin,mutagenesis,andmore. |
PackageContents | 20µllenti-EF1a-Cas9-EGFP-U6sgRNAVector(7.5ng/µl) |
PlasmidIdentity | -Vectoridentitywasconfirmedbysequencing.-A260/A280:1.8–2.0 |
Storage | Storeat-20°C.Avoidrepeatedfreeze/thawcycles.ShippedwithIceGelPacks. |
StorageBuffer | 10mMTris-HCl(pH8.0),1mMEDTA(pH8.0) |