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蚂蚁淘在线 / 品牌中心 / arp1 / American Research Products/AAV-U6 sgRNA-eGFP/Plasmid Only/CAS9-12-Plasmid Only
商品描述
ProductName | AAV-U6sgRNA-eGFP |
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Description | ThevectorAAV-U6sgRNA-EGFPisdesignedforexpressionofgRNAformammaliangenemodificationstudiesusingCRISPR/Cas9technologyinvivoorinvitro.sgRNAclonesexpressasingle-strandedchimericsgRNA,consistingofcrRNAandtracrRNA.Auser-definedsgRNAiscloneddownstreamofahumanU6promoter.Toconstructthevector,youmustconstructpGL3-U6sgRNA-Purowithyourguidesequence.TheU6-gRMAsequenceisobtainedbyPCR,andthePCRproductionisclonedintothepre-linearizedvectorusingtheincludedhigh-efficiencyligationmixandcompetentcells.InthepresenceoftheCas9endonuclease,sgRNAcanrecognizethetargetedDNAsequenceandguideCas9nucleaseduringgenomeeditingforgeneknockout,knockin,mutagenesis,andmore. |
PackageContents | 20µlAAV-U6sgRNA-EGFPVector(7.5ng/µl) |
PlasmidIdentity | -Vectoridentitywasconfirmedbysequencing.-A260/A280:1.8-2.0 |
Storage | Storeat-20°C.Avoidrepeatedfreeze/thawcycles.ShippedwithIceGelPacks. |
StorageBuffer | 10mMTris-HCl(pH8.0),1mMEDTA(pH8.0) |