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Chemical

Liquid.

Multi-species

Manufactured by Boston Biochem

Use: NEDD8 reaction buffer contains the optimal formulation for use in conjugation assays of the ubiquitin-like modifier NEDD8 to protein substrates in vitro. This requires the activities of the heterodimeric human E1 activating enzyme (APPBP1/Uba3) and the UbcH12 E2 enzyme. This buffer can be used to supplement NEDD8 conjugation kit. Dilute to 1X in final reaction.

BLUE ICE

+4°C

-20°C

Aliquot to avoid freeze/thaw cycles.

Stable for at least 1 year after receipt when stored at -20°C.

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NEDD8 (Neural precursor cell-expressed developmentally down-regulated gene 8; also known as Rub1) is a 6-8 kDa member of the ubiquitin family of proteins. It is expressed in striated muscle, and via its C-terminal Gly, forms covalent bonds with cullin family proteins. This conjugation generates ubiquitin ligase activity that regulates cell cycle proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. It contains one ubiquitin-like domain (aa 1-65), and a 4-5 aa C-terminal propeptide that is cleaved to expose a C-terminal Gly residue that is used to generate a Gly-Lys intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from both mouse and canine.

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