Product Specification:
| Alternative Name: | VIM |
| Clone: | TM71 |
| Host: | Rat |
| Isotype: | IgG2a |
| Immunogen: | Synthetic peptide corresponding to aa 66-77 of human Vimentin phosphorylated at Ser71. |
| UniProt ID: | P08670 |
| Species reactivity: | Human, Mouse, Rat |
| Applications: | ICC, WB |
| Recommended Dilutions/Conditions: | Immunocytochemistry (1µg/ml)Western Blot (1µg/ml for chemiluminescence detection system)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Application Notes: | Detects a band of ~58kDa by Western blot. |
| Purity Detail: | Protein G affinity purified. |
| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol. |
| Shipping: | Shipped on Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | Vimentin is one of four type II intermediate filament (IF) proteins; Desmin, Glial fibrillary acidic protein (GFAP) and Peripherin being the others. Vimentin is the most widely distributed of all IF proteins. It is typically expressed in leukocytes, blood vessel endothelial cells, and mesenchymal cells such as fibroblasts. Vimentin filaments help support cellular membranes. Vimentin networks also may help keep the nucleus and other organelles in a defined place within the cell. |
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