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UBB; Ubiquitin B

Protein

E. coli

Human ubiquitin (aa1-76) (Accession Nr. P0CG47) is site-specifically conjugated to a single Europium-Cryptate moiety. All lysines are available along with a fully functional C-terminus, making it ideal for chain formation using the E1-E2 and E3 conjugation cascade.

Human

Protein-Based TR-FRET Pair Donor. Typical working concentration range is 250nM-500nM. Best working concentration needs to be empirically determined by the type of application by the end user. The cryptate has an excitation maximum of 304nm and an emission maximum of 620nm.

Europium-Cryptate

~10kDa

≥98% (LCMS)

Lot dependent.

P0CG47

Liquid. In 50mM HEPES pH 7.5, 50mM sodium chloride.

Click here for a Typical Lot-specific Product Datasheet from the Original ManufacturerOur product description may differ slightly from the original manufacturers product datasheet.

Manufactured by South Bay Bio.

DRY ICE

-20°C

-80°C

Aliquot to avoid freeze/thaw cycles.Protect from light.

Stable for at least 1 year after receipt when stored at -80°C.

No

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Ubiquitin is a highly conserved protein that plays a major role in the ubiquitination pathway, which is conserved from yeast to mammals. Ubiquitination, the conjugation of ubiquitin to other proteins through a covalent bond between its C-terminal glycine and the ε-amino group of lysine residues or the α-amino group of an N- terminal methionine onto proteins is essential for many cellular processes primarily linked to protein degradation. This process involves three steps with specific groups of enzymes in an ATP depended manner, which are activation with ubiquitin-activating enzymes (E1s), conjugation with ubiquitin-conjugating enzymes (E2s) and ligation with ubiquitin ligases (E3s). This Europium Cryptate Ubiquitin is ideal for measuring Ub chain conjugation or deconjugation using TR-FRET measurement. Its TR-FRET pair is Cy5-Ubiquitin.

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