Pre-made lentiviral particles bicistronically express two individual proteins: nuclear permeable CRErecombinase and the RFP marker under the same enhanced EF1a promoter. CRE contains a NLS (nuclear localization signal)at its N-term. Thisnuclear permeantCREincreases the recombination events in vivo. A Blasticidin marker was expressed under RSV promoter. Particles allows to sort or select transduced cells for long term expression via RFP signal or via blasticidin antibiotic selection.

CRE and RFP are constitutively expressed in high level independent of cell types. RFP signal provides a convenient, realtime monitoring theparticles performance.

Cre protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences,named lox sites. CRE lentiviral particlescancatalyzethe jointing event In Vivoin the presence oflox sites. Purified CRE enzyme can joint individual plasmids containing lox sites.

Particles was concentrated and resuspended in PBS solution, best suitable for serum-free cultureorhard-to-transduced cell line,or direcly usedfor in vivo application.

See product manual for details (.pdf).

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