Pre-made lentiviral particlesexpressing sequence verified CRErecombinase underan enhanced EF1a promoter which is a strong promoter independent of tissue or cell types, and less likely to be silenced in long-term culture conditions.CRE contains a NLS (nuclear localization signal)at its N-term.Thisnuclear permeantCREincreases the recombination events in vivo. A blasticidin marker underRSV promoter, allows to select transduced cells for long term expression via blasticidin antibiotic.
Cre protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences,named lox sites. CRE lentiviral particlescancatalyzethe jointing event In Vivoin the presence oflox sites. Purified CRE enzyme can joint individual plasmids containing lox sites.
This product constitutively expresses nuclear localized CRE enzymein all cell types.This lentiviruswas purified into PBS solution which is used for non-serum cell culture and hard-to-transduced cell types, or direcly used for in vivo application.
See product manual for details (.pdf). Amount:200ul/per vial,5 x 107 IFU/ml in PBS. Cat#: LVP519-PBS
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