| Clone | 3EB |
| Isotype | IgG1 |
| Product Type | Single-Color Reagent |
| Units | 100 Tests |
| Host | Mouse |
| Species reactivity | Human |
| Application | Flow Cytometry |
BackgroundLFA-1betaisaleukocyteadhesionreceptorto(ICAM's)intercellularadhesion molecules.LFA-1 beta is on virtually all peripheral blood lymphocytes, 95,000 M.W. surface antigen.
Synonyms: CD18* FITC
Product
Product Form: FITC
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Purification Method: Protein A/G Chromatography
Concentration: Titered for flow cytometry
ApplicationsPBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS.Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Functional Analysis: Flow Cytometry Staining
StorageProduct should be stored at 4-8ºC. DO NOT FREEZE
Product Stability: Reagents are stable for the period shown on the vial label when stored properly
Shipping Conditions: Room Temperature
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References1. CD11c/CD18 on neutrophils recognizes a domain at the N terminus of the alpha chain of fibrinogen.Loike, J.D., Sodeik, B., Cao, L., Leucona, S., Weitz, J.I., Detmers, P.A., Wright, S.D., Silverstein, S.C.. Proc. Natl. Acad. Sci. USA, 1991 Feb; 88(3):1044-8. 2. T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1.Dustin, M.L. and Springer, T.A..Nature. 1989:341:619. 3. Coupling of the adhesive receptor CD11/CD18 to functional enhancement of effector macrophage tissue factor response. Fan, S.T. and Edgington, T.S..J. Clin. Invest. 1991 Ja:87(1):50-7. 4. Leukocyte Adhesion Deficiency: an inherited defect in Mac-1,LFA-1, and p150,95 glycoproteins.Anderson, D.C. and Springer, T.A.. Ann. Rev. Med.1987;38:175. 5. Two pathways of CD11b/CD18-mediated neutrophil aggregation with different involvement of protein kinase C-dependent phosphorylation. Merrill, J.T., Slade, S.G., Weissman, G., Winchester, R., Buyon, J.P..J. Immunology. 1990 Oct.1;145(8):2608-15. 6. Tumor necrosis factor-induce degranulation in adherent human neutrophils is dependent on CD11b/CD18-integrin -triggered oscillations of cytosolic free Ca2+.Richter, J., Ng-Sikorski, J., Olsson, I., Andersson, T..Proc. Natl. Acad. Sci. USA, 1990 De;87(23):9472-6.
Protein Reference(s)
Database Name: UniProt
Accession number: P05107
Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf
