| Clone | ARE |
| Isotype | IgM |
| ProductType | Single-ColorReagent |
| Units | 100µg |
| Host | Mouse |
| Speciesreactivity | Human |
| Application | FlowCytometry |
BackgroundIdentificationofCD15thatrecognizesahumanmylelomonocyticantigen.ThestructurerecognizedbyCD15antibodiesislacto-N-fucopentoseIII.1.TheCD15antigenispresentongreaterthan95%ofmatureperipheralbloodeosinophilsandneutrophils.Itispresentatlowdensityoncirculatingmonocytes.InlymphoidtissueCD15reactswithReed-SternbergcellsofHodgkinsdiseaseandwithgranulocytes.However,CD15reactswithonlyafewtissuemacrophagesanddoesnotreactwithdendriticcells.
Synonyms:CD15
Product
ProductForm:Unconjugated
Formulation:Providedassolutioninphosphatebufferedsalinewith0.08%sodiumazide
PurificationMethod:ProteinA/GChromatography
Concentration:Seevialforconcentration
ApplicationsPBMC:Add10µlofMAB/10^6PBMCin100µlPBS.Mixgentlyandincubatefor15minutesat2ºto8ºC.WashtwicewithPBSandanalyzeorfixwith0.5%v/vofparaformaldehydeinPBSandanalyze.WHOLEBLOOD:Add10µlofMAB/100µlofwholeblood.Mixgentlyandincubatefor15minutesatroomtemperature(20ºC).Lysethewholeblood.WashoncewithPBSandanalyzeorfixwith0.5%v/vofparaformaldehydeinPBSandanalyze.Seeinstrumentmanufacturer’sinstructionsforLysedWholeBloodandImmunofluorescenceanalysiswithaflowcytometerormicroscope.
FunctionalAnalysis:FlowCytometryStaining
StorageProductshouldbestoredat-20ºC.Aliquottoavoidfreeze/thawcycles
ProductStABIlity:Productsarestableforoneyearfrompurchasewhenstoredproperly
ShippingConditions:RoomTemperature
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.
References1.SkubitzK,BalkeJ,BallE,etal.ReportontheCD15clusterworkshop.In:KnappW,DörkenB,GilksWR,etal,eds.LeucocyteTypingIV:WhiteCellDifferentiationAntigens.Oxford:OxfordUniversityPress;1989;800-805.2.HanjanSNS,KearneyJF,CooperMD.Amonoclonalantibody(MMA)thatidentifiesadifferentiationantigenonhumanmyelomonocyticcells.ClinImmunolImmunopath.1982;23:172.3.HsuSM,JaffeES.Leu-M1andpeanutagglutininstaintheneoplasticcellsofHodgkin"sDisease.AmerJClinPath.1984;82:29.4.PinkusGS,ThomasP,SaidJW.Leu-M1—AMarkerforReedSternbergcellsinHodgkin"sDisease:Animmunoperoxidasestudyofparaffin-embeddedtissues.AmJPathol.1985;119:244.
SafetyDatasheet(s)forthisproduct:NM_SodiumAzide/wp-content/uploads/SDS/AntibodySDSwithSodiumAzideNoridic-MUbio.pdf
Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。
