| Isotype | IgG |
| Product Type | Polyclonal Antibody |
| Units | 100 µg |
| Host | Rabbit |
| Species reactivity | Human |
| Application | Western Blotting |
BackgroundMembers in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3 , and Nix (for Nip3-like protein X) (1-3). Bnip3L/Bnip3 /Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis (2,3). Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (1,2). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.
Synonyms: BNIP3A, BNIP3H, NIX
Source
Immunogen: Synthetic peptide corresponding to amino acids 77 to 92 of the human Bnip3L protein.Immunogen sequence is identical in the mouse protein.
Product
Product Form: Unconjugated
Formulation: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative
Purification Method: Antigen Immunoaffiinity Purification
Concentration: See vial for concentration
ApplicationsDetects Bnip3L by Western blot at 0.5 to 1 µg/ml.Detects a 40 kDa band in K562 cell lysate which represents the monomer of Bnip3L.Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: K562 cell lysate
StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References1. Matsushima, M., et al., Isolation, mapping, and functional analysis of a novel human cDNA (BNIP3L) encoding a protein homologous to human NIP3. Genes Chromosomes Cancer 1998, 21, 230-2352. Yasuda, M., et al., BNIP3alpha: a human homolog of mitochondrial proapoptotic protein BNIP3. Cancer Res. 1999, 59, 533-5373. Chen, G., et al., Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins. J. Biol. Chem. 1999, 274, 7-104. Imazu, T., et al., Bcl-2/E1B 19 kDa-interacting protein 3-like protein (Bnip3L) interacts with bcl-2/Bcl-xL and induces apoptosis by altering mitochondrial membrane permeability. Oncogene. 1999, 18, 4523-4529Product Specific References: 1. Aerbajinai, W., et al. 'The proapoptotic factor Nix is coexpressed with Bcl-xL during terminal erythroid differentiation.' Blood, 2003, 102, 712-717.2. Zhang, J., et al. 'Mitochondrial clearance is regulated by Atg7-dependent and -independent mechanisms during reticulocyte maturation.' Blood, 2009, 114, 157-164.
Protein Reference(s)
Database Name: UniProt
Accession number: O60238
Species Accession: Human
Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf
