| Isotype | IgG |
| ProductType | PolyclonalAntibody |
| Units | 100µg |
| Host | Rabbit |
| Speciesreactivity | Human |
| Application | WesternBlotting |
BackgroundVoltage-dependentcalciumchannels(VDCCs)arelarge(>400kDa)heteromerswhichcontain,minimally,threecoresubunits1,2/,ina1:1:1stoichiometry1.ExpressionofVDCCgeneproductsinXenopusoocytes,ortransfectedcellsshowsthatthe1subunitscontaintheionchannelporewhiletheauxiliary2/andsubunitsconferoptimalcellsurfaceexpressionandchannelkinetics1.Untilrecently,theonlyexceptiontotheaboveparADIgmwastheskeletalmuscleVDCC,which,inadditiontothe1,2/,coremotif,alsohasanadditionaltightlyassociatedintegralmembraneglycoproteinsubunittermed1.Uponco-expressionwiththe1.1,2/1,1asubunitsoftheskeletalmuscleVDCC,subunitsalterthepeakcurrents,andthekineticsofchannelactivationandinactivationwiththeoveralleffectbeinganormalisationofcurrentstothoseresemblingtheendogenouschannel2.Together,theseresultssuggestthatsubunitsmodulateskeletalmuscleVDCCsbystABIlisingtheirconformation.The2subunits,orstargazin,theproductofagenemutatedinthestargazermouse,isahomologofthe1channelandisselectivelyexpressedinthebrain.ItisconsideredtobeaputativeneuronalCa2+channelsubtypemainlybasedonitshomologytothe1.
Synonyms:Neuronalvoltage-gatedcalciumchannelgamma-2subunit;TransmembraneAMPARregulatoryproteingamma-2;Voltage-dependentcalciumchannelgamma-2subunit
Source
Immunogen:Syntheticpeptidederivedfromtheratcalciumchannelgamma2subunitconjugatedtoKLH
Product
ProductForm:Unconjugated
Formulation:Providedassolutioninphosphatebufferedsalinewith0.08%sodiumazide
PurificationMethod:AmmoniumSulfatePrecipitation
Concentration:Seevialforconcentration
ApplicationsThisantibodycanbeusedforWesternblottingat5-10µg/ml.Optimalconcentrationshouldbeevaluatedbyserialdilutions.
FunctionalAnalysis:WesternBlotting
PositiveControl:Ratbrainlysate
StorageProductshouldbestoredat-20ºC.Aliquottoavoidfreeze/thawcycles
ProductStability:Productsarestableforoneyearfrompurchasewhenstoredproperly
ShippingConditions:Shipatambienttemperature,freezeuponarrival
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.
References1.HofmannF,etal.RevPhysiolBiochemPharmacol1999,139:33-872.SingerD,etal.Science1991,253:1553-73.LettsVA,etal.NatGenet1998,19:340-74.Kang,M.G.,etal.J.Biol.Chem.2001,276:32917-245.Sharp,A.H.,etal.Neuroscience2001,105:599-6176.SteinleinOK,NoebelsJLCurrOpinGenetDev2000,10:286-917.Chen,L.,etal."StargazinregulatessynaptictargetingofAMPAreceptorsbytwodistinctmechanisms."Nature2000,408:936-943.
ProteinReference(s)
DatabaseName:SwissProt
Accessionnumber:Q9Y698
SpeciesAccession:Human
SafetyDatasheet(s)forthisproduct:NM_SodiumAzide/wp-content/uploads/SDS/AntibodySDSwithSodiumAzideNoridic-MUbio.pdf
Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。
