| Isotype | IgG |
| ProductType | PhosphorylationSite-SpecificAntibody |
| Units | 100µg |
| Host | Rabbit |
| Speciesreactivity | Human |
| Application | EnzymeImmunoassayImmunohistochemistryWesternBlotting |
BackgroundVarianthistoneH2AwhichreplacesconventionalH2Ainasubsetofnucleosomes.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.Requiredforcheckpoint-mediatedarrestofcellcycleprogressioninresponsetolowdosesofionizingrADIationandforefficientrepairofDNAdoublestrandbreaks(DSBs)specificallywhenmodifiedbyC-terminalphosphorylation.
Synonyms:H2AFX;H2AX
Source
Immunogen:PhosphopeptidederivedfromthehumanhistoneH2A.xprotein.
Product
ProductForm:AffinityPurified
Formulation:Providedassolutioninphosphatebufferedsalinewith0.08%sodiumazide
PurificationMethod:AntigenImmunoaffiinityPurification
Concentration:Seevialforconcentration
ApplicationsAntibodycanbeusedforWesternblotting(1-5µg/ml)andimmunohistochemistry(2-5µg/mlatpH6.2).Optimalconcentrationshouldbeevaluatedbyserialdilutions.
FunctionalAnalysis:WesternBlotting
PositiveControl:Humanbladder
StorageProductshouldbestoredat-20ºC.Aliquottoavoidfreeze/thawcycles
ProductStability:Productsarestableforoneyearfrompurchasewhenstoredproperly
ShippingConditions:Shipatambienttemperature,freezeuponarrival
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.
References1.Paull,T.T.,etal.‘AcriticalroleforhistoneH2AXinrecruitmentofrepairfactorstonuclearfociafterDNAdamage’Curr.Biol.,10,886-895(2000)2.Kobayashi,J.,etal.‘NBS1localizestogamma-H2AXfocithroughinteractionwiththeFHA/BRCTdomain.’Curr.Biol.,12,1846-1851(2002)3.Stewart,G.S.,etal.‘MDC1isamediatorofthemammalianDNAdamagecheckpoint.’Nature,421,961-966(2003)4.Lukas,C.,etal.‘Mdc1couplesDNAdouble-strandbreakrecognitionbyNbs1withitsH2AX-dependentchromatinretention.’EMBOJ.,23,2674-2683(2004)
ProteinReference(s)
DatabaseName:SwissProt
Accessionnumber:P16104
SpeciesAccession:Human
SafetyDatasheet(s)forthisproduct:NM_SodiumAzide/wp-content/uploads/SDS/AntibodySDSwithSodiumAzideNoridic-MUbio.pdf
Nordic的纯化的牛IgG可以在各种免疫测定（包括免疫扩散，免疫电泳，ELISA，Western印迹，斑点免疫结合测定（DIBA），血细胞凝集和细胞结合测定）中用作参考抗原，校准物，包被蛋白和封闭剂。该产品仅用于研究目的，并且用于体外测试，不适用于涉及人或动物的诊断或治疗程序。它可能包含有害成分。请参阅安全数据表（SDS）了解更多信息和正确的处理程序。根据当地法规处理产品残留物。此数据表的准确性尽可能合理，但Nordic-MUbio对本信息中的任何准确性或遗漏概不负责。
