| Clone | 2B6 |
| Isotype | IgG1 |
| Product Type | Monoclonal Antibody |
| Units | 100 µg |
| Host | Mouse |
| Species reactivity | HumanRat |
| Application | ImmunocytochemistryImmunohistochemistryWestern Blotting |
BackgroundCadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. Cadherin-6 is expressed in the proximal tubule epithelium of the kidney from which most renal cell carcinomas are derived.
Synonyms: K-Cadherin
Source
Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with affinity purified extracellular domain of human cadherin-6 fusion protein and SP2/0 mouse myeloma cells.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
ApplicationsAntibody can be used for Western blotting (1:25-1:250), immunocytochemistry and immunohistochemistry on frozen sections using a PBS buffer containing 0.1 mM CaCl2and 0.1 mM MgCl2.Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References1. Shimazui, T., et al. 'Expression of cadherin-6 as a novel diagnostic tool to predict prognosis of patients with E-cadherin-absent renal cell carcinoma,' Clin Cancer Res 1998, 4, 2419-24.2. Shimazui, T., et al. 'Alterations in expression of cadherin-6 and Ecadherin during kidney development and in renal cell carcinoma,' Eur Urol 2000, 38, 331-8.
Protein Reference(s)
Database Name: SwissProt
Accession number: P55285
Species Accession: Human
Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf
